11-750-550
Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-750-550-Each-of-1 | In Stock | ₹ 2,14,755.60 |
11-750-550 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,14,755.60
GST (18%): ₹ 38,656.008
Total Price: ₹ 2,53,411.608
Content And Storage
• SuperScript IV CellsDirect Lysis Solution (9 mL)• SuperScript IV CellsDirect Stop Solution (1.25 mL)• Lysis Enhancer (250 μL)Store at –20°C.
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript IV
No. of Reactions
500 reactions
Reagent Type
Reverse Transcription
Format
Kit
For Use With
SuperScript IV CellsDirect cDNA Synthesis Kit
Shipping Condition
Dry Ice
Reaction Format
Master Mix
Starting Material
Cells
Related Products
Description
- The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer
- These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples
- See the SuperScript IV CellsDirect cDNA Synthesis Kit manual for protocol recommendations
- SuperScript IV CellsDirect Lysis Reagents are optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating RNA
- Features of SuperScript IV CellsDirect Lysis Reagents include: • Ready-to-use pre-mixed cell lysis solution compatible with a variety of cell-types • Optimized performance for 1–10,000 cells per sample; results equivalent to those from purified RNA Note: This procedure is optimized for a maximum of 10,000 cells per reaction
- If needed, volumes can be adjusted for cell numbers greater than 10,000
- Using too many cells per lysis reaction can result in incomplete lysis and/or inhibition of RT-PCR.
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Content And Storage: • SuperScript IV CellsDirect Lysis Solution (9 mL)• SuperScript IV CellsDirect Stop Solution (1.25 mL)• Lysis Enhancer (250 μL)Store at –20°C.
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript IV
No. of Reactions: 500 reactions
Reagent Type: Reverse Transcription
Format: Kit
For Use With: SuperScript IV CellsDirect cDNA Synthesis Kit
Shipping Condition: Dry Ice
Reaction Format: Master Mix
Starting Material: Cells
Content And Storage:
• SuperScript IV CellsDirect Lysis Solution (9 mL)• SuperScript IV CellsDirect Stop Solution (1.25 mL)• Lysis Enhancer (250 μL)Store at –20°C.
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript IV
No. of Reactions:
500 reactions
Reagent Type:
Reverse Transcription
Format:
Kit
For Use With:
SuperScript IV CellsDirect cDNA Synthesis Kit
Shipping Condition:
Dry Ice
Reaction Format:
Master Mix
Starting Material:
Cells
Content And Storage: • 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
Technique: Reverse Transcription, Preamplification
Reverse Transcriptase: SuperScript IV
No. of Reactions: 48 Reactions
Reagent Type: Reverse Transcription
Format: Kit
For Use With: __
Shipping Condition: Dry Ice
Reaction Format: __
Starting Material: Cells or RNA
Content And Storage:
• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
Technique:
Reverse Transcription, Preamplification
Reverse Transcriptase:
SuperScript IV
No. of Reactions:
48 Reactions
Reagent Type:
Reverse Transcription
Format:
Kit
For Use With:
__
Shipping Condition:
Dry Ice
Reaction Format:
__
Starting Material:
Cells or RNA
Content And Storage: • 2X RT Reaction Mix (500 μL)• RT Enzyme Mix (100 μL)•E. coliRNase (50 μL)Store at –20°C.
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Format: Super Mix
For Use With: __
Shipping Condition: Dry Ice
Reaction Format: Master Mix
Starting Material: RNA
Content And Storage:
• 2X RT Reaction Mix (500 μL)• RT Enzyme Mix (100 μL)•E. coliRNase (50 μL)Store at –20°C.
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Format:
Super Mix
For Use With:
__
Shipping Condition:
Dry Ice
Reaction Format:
Master Mix
Starting Material:
RNA
Content And Storage: Two packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
Technique: Reverse Transcription, Preamplification
Reverse Transcriptase: SuperScript IV
No. of Reactions: 96 Reactions
Reagent Type: Reverse Transcription
Format: Kit
For Use With: __
Shipping Condition: Dry Ice
Reaction Format: __
Starting Material: Cells or RNA
Content And Storage:
Two packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
Technique:
Reverse Transcription, Preamplification
Reverse Transcriptase:
SuperScript IV
No. of Reactions:
96 Reactions
Reagent Type:
Reverse Transcription
Format:
Kit
For Use With:
__
Shipping Condition:
Dry Ice
Reaction Format:
__
Starting Material:
Cells or RNA