K310002
Invitrogen™ PureLink™ PCR Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K310002-Each-of-1 | In Stock | ₹ 50,822.64 |
K310002 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,822.64
GST (18%): ₹ 9,148.075
Total Price: ₹ 59,970.715
Content And Storage
• 72 mL Binding Buffer B2; room temperature• 109 mL Binding Buffer High-Cutoff B3; room temperature• 80 mL Wash Buffer W1; room temperature• 15 mL Elution Buffer; room temperature• 5 x 50 PureLink PCR Spin Columns with Collection Tubes; room temperature• 5 x 50 PureLink Elution Tubes; room temperature
Elution Volume Max
50 μL
Purification Time
15 min.
Run Time
10 min.
Starting Material Volume
≤100 μL PCR product, or ≤40 μg dsDNA
For Use With (Application)
Standard PCR, DNA Sequencing, Restriction Enzyme Digestion, Nucleic Acid Labeling, Cloning
Isolation Technology
Silica Spin Column
Format
Kit
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
250 Preps
Shipping Condition
Room Temperature
Yield
40 μg (Binding capacity)
Final Product Type
PCR Amplicon
Sample Type
PCR Products
Related Products
Description
- The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits
- After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments
- Advantages of the PureLink PCR Purification Kit include: • 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits • No need for tedious sample pH adjustments necessary with other kits • Convenient and rapid protocol, completed in less than 10 minutes • Optional size-selection buffers included for selectively purifying 300–600 bp fragments The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts
- Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column
- The dsDNA binds to the silica-based membrane in the column
- Remove impurities by thorough washing with Wash Buffer
- To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning
- Order Info Shipping Condition: Room Temperature
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Content And Storage: • 72 mL Binding Buffer B2; room temperature• 109 mL Binding Buffer High-Cutoff B3; room temperature• 80 mL Wash Buffer W1; room temperature• 15 mL Elution Buffer; room temperature• 5 x 50 PureLink PCR Spin Columns with Collection Tubes; room temperature• 5 x 50 PureLink Elution Tubes; room temperature
Elution Volume Max: 50 μL
Purification Time: 15 min.
Run Time: 10 min.
Starting Material Volume: ≤100 μL PCR product, or ≤40 μg dsDNA
For Use With (Application): Standard PCR, DNA Sequencing, Restriction Enzyme Digestion, Nucleic Acid Labeling, Cloning
Isolation Technology: Silica Spin Column
Format: Kit
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: 40 μg (Binding capacity)
Final Product Type: PCR Amplicon
Sample Type: PCR Products
Content And Storage:
• 72 mL Binding Buffer B2; room temperature• 109 mL Binding Buffer High-Cutoff B3; room temperature• 80 mL Wash Buffer W1; room temperature• 15 mL Elution Buffer; room temperature• 5 x 50 PureLink PCR Spin Columns with Collection Tubes; room temperature• 5 x 50 PureLink Elution Tubes; room temperature
Elution Volume Max:
50 μL
Purification Time:
15 min.
Run Time:
10 min.
Starting Material Volume:
≤100 μL PCR product, or ≤40 μg dsDNA
For Use With (Application):
Standard PCR, DNA Sequencing, Restriction Enzyme Digestion, Nucleic Acid Labeling, Cloning
Isolation Technology:
Silica Spin Column
Format:
Kit
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
40 μg (Binding capacity)
Final Product Type:
PCR Amplicon
Sample Type:
PCR Products
Content And Storage: Store at room temperature.
Elution Volume Max: __
Purification Time: __
Run Time: __
Starting Material Volume: __
For Use With (Application): __
Isolation Technology: __
Format: __
High-throughput Compatibility: __
Quantity: 120 mL
Shipping Condition: Room Temperature
Yield: __
Final Product Type: __
Sample Type: __
Content And Storage:
Store at room temperature.
Elution Volume Max:
__
Purification Time:
__
Run Time:
__
Starting Material Volume:
__
For Use With (Application):
__
Isolation Technology:
__
Format:
__
High-throughput Compatibility:
__
Quantity:
120 mL
Shipping Condition:
Room Temperature
Yield:
__
Final Product Type:
__
Sample Type:
__
Content And Storage: Store the wash buffer at room temperature
Elution Volume Max: __
Purification Time: __
Run Time: __
Starting Material Volume: __
For Use With (Application): __
Isolation Technology: __
Format: __
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 40 mL
Shipping Condition: Room Temperature
Yield: __
Final Product Type: DNA
Sample Type: PCR Products
Content And Storage:
Store the wash buffer at room temperature
Elution Volume Max:
__
Purification Time:
__
Run Time:
__
Starting Material Volume:
__
For Use With (Application):
__
Isolation Technology:
__
Format:
__
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
40 mL
Shipping Condition:
Room Temperature
Yield:
__
Final Product Type:
DNA
Sample Type:
PCR Products
Content And Storage: Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Elution Volume Max: 20 μL
Purification Time: __
Run Time: 10 min.
Starting Material Volume: 125 μL
For Use With (Application): Cloning, Standard PCR
Isolation Technology: Silica Spin Column
Format: Spin Column
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Shipping Condition: Room Temperature
Yield: __
Final Product Type: __
Sample Type: cDNA Labeling Reactions
Content And Storage:
Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Elution Volume Max:
20 μL
Purification Time:
__
Run Time:
10 min.
Starting Material Volume:
125 μL
For Use With (Application):
Cloning, Standard PCR
Isolation Technology:
Silica Spin Column
Format:
Spin Column
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Shipping Condition:
Room Temperature
Yield:
__
Final Product Type:
__
Sample Type:
cDNA Labeling Reactions