FERK0702
Thermo Scientific™ GeneJET PCR Purification Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | FERK0702-Each-of-1 | In Stock | ₹ 43,592.82 |
FERK0702 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,592.82
GST (18%): ₹ 7,846.708
Total Price: ₹ 51,439.528
Content And Storage
• 60 mL Binding Buffer; room temperature• 45 mL Wash Buffer (concentrated); room temperature• 30 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature
Elution Volume Max
50 μL
Purification Time
5 min.
Run Time
5 min.
Starting Material Volume
≤400 μL
For Use With (Application)
Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization
Isolation Technology
Silica Spin Column
Format
Spin Column
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
250 Preps
Shipping Condition
Room Temperature
Yield
25 μg (Binding capacity)
Final Product Type
DNA
Sample Type
PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Related Products
Description
- Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions
- It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures
- The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%
- Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes
- The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization
- Highlights • Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb • Fast: procedure takes only 5 minutes • Convenient: spin columns are capped and assembled with collection tubes Applications Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including: • FastDigest or conventional restriction digestion • Automated fluorescent or radioactive sequencing • PCR • In vitro transcription • In situ hybridization • Labeling • Cloning
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Content And Storage: • 60 mL Binding Buffer; room temperature• 45 mL Wash Buffer (concentrated); room temperature• 30 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature
Elution Volume Max: 50 μL
Purification Time: 5 min.
Run Time: 5 min.
Starting Material Volume: ≤400 μL
For Use With (Application): Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization
Isolation Technology: Silica Spin Column
Format: Spin Column
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: 25 μg (Binding capacity)
Final Product Type: DNA
Sample Type: PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Content And Storage:
• 60 mL Binding Buffer; room temperature• 45 mL Wash Buffer (concentrated); room temperature• 30 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature
Elution Volume Max:
50 μL
Purification Time:
5 min.
Run Time:
5 min.
Starting Material Volume:
≤400 μL
For Use With (Application):
Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization
Isolation Technology:
Silica Spin Column
Format:
Spin Column
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
25 μg (Binding capacity)
Final Product Type:
DNA
Sample Type:
PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Content And Storage: 60 mL Binding Buffer, 45 mL Wash Buffer (concentrated), 30 mL Elution Buffer, 250 GeneJET Purification Columns with Collection Tubes
Elution Volume Max: 50 μL
Purification Time: 5 min.
Run Time: 5 min.
Starting Material Volume: ≤400 μL
For Use With (Application): Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization
Isolation Technology: Silica Spin Column
Format: Spin Column
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: 25 μg (Binding capacity)
Final Product Type: DNA
Sample Type: PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Content And Storage:
60 mL Binding Buffer, 45 mL Wash Buffer (concentrated), 30 mL Elution Buffer, 250 GeneJET Purification Columns with Collection Tubes
Elution Volume Max:
50 μL
Purification Time:
5 min.
Run Time:
5 min.
Starting Material Volume:
≤400 μL
For Use With (Application):
Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization
Isolation Technology:
Silica Spin Column
Format:
Spin Column
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
25 μg (Binding capacity)
Final Product Type:
DNA
Sample Type:
PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Content And Storage: __
Elution Volume Max: __
Purification Time: __
Run Time: __
Starting Material Volume: __
For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
Isolation Technology: Spin Column
Format: Spin Column
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Shipping Condition: __
Yield: __
Final Product Type: __
Sample Type: Bacteria, Buccal Samples, Cells, Tissue, Yeast
Content And Storage:
__
Elution Volume Max:
__
Purification Time:
__
Run Time:
__
Starting Material Volume:
__
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
Isolation Technology:
Spin Column
Format:
Spin Column
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Shipping Condition:
__
Yield:
__
Final Product Type:
__
Sample Type:
Bacteria, Buccal Samples, Cells, Tissue, Yeast
Content And Storage: • 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening• 55 mL Digestion Solution; room temperature• 2 x 60 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Elution Volume Max: __
Purification Time: __
Run Time: __
Starting Material Volume: __
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Isolation Technology: Silica Spin Column
Format: __
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: ≤30 μg
Final Product Type: gDNA
Sample Type: Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
Content And Storage:
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening• 55 mL Digestion Solution; room temperature• 2 x 60 mL Lysis Solution; room temperature• 40 mL Wash Buffer I (concentrated); room temperature• 40 mL Wash Buffer II (concentrated); room temperature• 150 mL Elution Buffer; room temperature• 250 GeneJET Purification Columns with Collection Tubes; room temperature• 250 Collection Tubes; room temperature
Elution Volume Max:
__
Purification Time:
__
Run Time:
__
Starting Material Volume:
__
For Use With (Application):
PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
Isolation Technology:
Silica Spin Column
Format:
__
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
≤30 μg
Final Product Type:
gDNA
Sample Type:
Bacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples