43-511-07
Applied Biosystems™ 7500 Fast Real-Time PCR System, desktop
Manufacturer: Applied Biosystems™
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Format
96-well Plate, 8-well Strips
Sample Ramp Rate
Fast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
Temperature Range (Metric)
4.0 to 99.9°C
Passive Reference Dye
ROX (Separate Tube), Other Passive Reference Dye, ROX (Pre-mixed)
Content And Storage
Desktop Computer
For Use With (Equipment)
7500 Fast System
Reaction Speed
Fast, Standard
Run Time
36 min. (Fast), <2 hrs (Standard)
Thermal Cycling System
Peltier-based System
Capacity
12 x 8-tube Strips, 1 x 96-well Plate (Fast)
Precision
Distinguish between 5000 to 10,000 genome equivalents (two-fold Copy Number difference) with 99.7% Confidence
Max. Heating Rate (Thermal Block)
5.5°C
Weight
34 kg (75 lb.)
Thermal Accuracy
±0.5°C
Sensitivity
Down to 1 Copy of Human RNAse P gene
Reaction Volume Range
10-30 μl
Dynamic Range
9 Logs of Linear Dynamic Range
Calibrated Dye
TAMRA, Cy3, FAM, SYBR Green, Texas Red, JOE, ROX, Cy5, NED, VIC
Thermal Uniformity
±1°C
Throughput
4 to 5 96-well Plates per day
Optics
CCD Camera, Halogen Light Source, Five Excitation and Five Emission Filters
Dimensions
34 cm (13.39 in.) (W) x 49 cm (19.29 in.) (H) x 45 cm (17.72 in.) (D)
External Computer
Computer Tower Provided
Peak Block Ramp Rate
5.5°C⁄s
Quantity
1 System
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Description
- The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time
- Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes
- We supply a Dell™ Tower computer with the 7500 Fast System
- Specially designed Fast block ensures thermal uniformity at top speeds
- Faster ramp rates enable rapid results without compromising extension times or assay quality
- Fast optical plates ensure excellent precision in 10 to 30μL reaction volumes
- Use TaqMan™ Fast Universal PCR Master Mix to run multiple Fast assays on one plate
- Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System
- High Resolution Melting Analysis : The Applied Biosystems™ High Resolution Melting (HRM) Software is the easiest to use melting analysis software enabling real-time PCR melt curve assays to be used more accurately for mutation scanning and genotyping
- The HRM Software is available on the 7500 Fast Real-Time PCR System, which delivers precise results in a standard 96-well format
- Use the Applied Biosystems™ High Resolution Melting (HRM) Software to perform more sophisticated melting analysis with an easy to follow workflow and minimal subjective data analysis steps
- Shortens analysis time by auto-calling genotypes and automatically omitting the no template controls
- Minimizes subjective analysis by automatically grouping unknown variant clusters
- No temperature shift required – distinguish between homozygous mutants and wild type more easily
- Allows easy data review with customizable multi-plot views, expandable windows and one-click color assignment to highlight curves of interest
- Facilitates data presentation with the option to export data and graphs directly to PowerPoint or as JPEG files
- NEW - 7500 Software v2.0: Now the easy-to-use StepOne software is available for both the 7500 and 7500 Fast systems with the 7500 Software v2.0 upgrade
- The 7500 Software v2.0 incorporates your favorite StepOne Software features, such as a variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification when a run is finished
- The 7500 Software v2.0 also includes an enhanced Gene Expression Study package and has a variety of new melting curve protocol options, including multiple peak detection, step and hold temperature control, and customizable ramp rates
- The Gene Expression Study package accommodates large studies better than any other instrument software package: Import an unlimited number of Comparative CT (relative quantitation) files to one study
- Group samples and view data both by technical replicate group and biological replicate group
- Use any gene(s) as an endogenous control, including averaging multiple controls together
- Enter known efficiency values to be factored into the RQ results 21 CFR Part 11 Module available : The SDS v1.4 21CFRp11 Module is a powerful tool for assisting with 21CFRp11 compliance, while still offering the flexibility of user-customizable configuration settings
- Individual user log-ins can be added for up to four user groups, each group with designated permission settings
- User-customizable permission settings include fourteen system activities, e-signature authority designation, and additional security settings to give you maximum control over your compliance efforts
- Audit trails can be enabled or disabled depending on your traceability needs
- A selection of e-signatures is available to ease e-signatures into your workflow
- One System, Many Applications: Applications include gene expression analysis, pathogen quantitation, SNP genotyping, isothermal and +⁄– assays utilizing internal positive controls
- To facilitate many of these applications, Applied Biosystems™ provides preformulated, ready-to-use, quality-tested, TaqMan™ assays for use with the 7500 Fast system
- Now you can reduce your assay optimization efforts
- For Research Use Only
- Not for use in diagnostics procedures.
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Format: 96-well Plate, 8-well Strips
Sample Ramp Rate: Fast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
Temperature Range (Metric): 4.0 to 99.9°C
Passive Reference Dye: ROX (Separate Tube), Other Passive Reference Dye, ROX (Pre-mixed)
Content And Storage: Desktop Computer
For Use With (Equipment): 7500 Fast System
Reaction Speed: Fast, Standard
Run Time: 36 min. (Fast), <2 hrs (Standard)
Thermal Cycling System: Peltier-based System
Capacity: 12 x 8-tube Strips, 1 x 96-well Plate (Fast)
Precision: Distinguish between 5000 to 10,000 genome equivalents (two-fold Copy Number difference) with 99.7% Confidence
Max. Heating Rate (Thermal Block): 5.5°C
Weight: 34 kg (75 lb.)
Thermal Accuracy: ±0.5°C
Sensitivity: Down to 1 Copy of Human RNAse P gene
Reaction Volume Range: 10-30 μl
Dynamic Range: 9 Logs of Linear Dynamic Range
Calibrated Dye: TAMRA, Cy3, FAM, SYBR Green, Texas Red, JOE, ROX, Cy5, NED, VIC
Thermal Uniformity: ±1°C
Throughput: 4 to 5 96-well Plates per day
Optics: CCD Camera, Halogen Light Source, Five Excitation and Five Emission Filters
Dimensions: 34 cm (13.39 in.) (W) x 49 cm (19.29 in.) (H) x 45 cm (17.72 in.) (D)
External Computer: Computer Tower Provided
Peak Block Ramp Rate: 5.5°C⁄s
Quantity: 1 System
Format:
96-well Plate, 8-well Strips
Sample Ramp Rate:
Fast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
Temperature Range (Metric):
4.0 to 99.9°C
Passive Reference Dye:
ROX (Separate Tube), Other Passive Reference Dye, ROX (Pre-mixed)
Content And Storage:
Desktop Computer
For Use With (Equipment):
7500 Fast System
Reaction Speed:
Fast, Standard
Run Time:
36 min. (Fast), <2 hrs (Standard)
Thermal Cycling System:
Peltier-based System
Capacity:
12 x 8-tube Strips, 1 x 96-well Plate (Fast)
Precision:
Distinguish between 5000 to 10,000 genome equivalents (two-fold Copy Number difference) with 99.7% Confidence
Max. Heating Rate (Thermal Block):
5.5°C
Weight:
34 kg (75 lb.)
Thermal Accuracy:
±0.5°C
Sensitivity:
Down to 1 Copy of Human RNAse P gene
Reaction Volume Range:
10-30 μl
Dynamic Range:
9 Logs of Linear Dynamic Range
Calibrated Dye:
TAMRA, Cy3, FAM, SYBR Green, Texas Red, JOE, ROX, Cy5, NED, VIC
Thermal Uniformity:
±1°C
Throughput:
4 to 5 96-well Plates per day
Optics:
CCD Camera, Halogen Light Source, Five Excitation and Five Emission Filters
Dimensions:
34 cm (13.39 in.) (W) x 49 cm (19.29 in.) (H) x 45 cm (17.72 in.) (D)
External Computer:
Computer Tower Provided
Peak Block Ramp Rate:
5.5°C⁄s
Quantity:
1 System
Format: __
Sample Ramp Rate: __
Temperature Range (Metric): __
Passive Reference Dye: __
Content And Storage: Contains three Optical 96-Well Reaction Plates, preloaded and sealed containing Cy3™ Texas Red™ and Cy5™ fluorescent dye standards.Store at -15 to -25°C. (This product is light sensitive.)Expiration date: 12 months from the date of delivery
For Use With (Equipment): 7500 System
Reaction Speed: __
Run Time: __
Thermal Cycling System: __
Capacity: __
Precision: __
Max. Heating Rate (Thermal Block): __
Weight: __
Thermal Accuracy: __
Sensitivity: __
Reaction Volume Range: __
Dynamic Range: __
Calibrated Dye: Cy™3, Cy™5, Texas Red™
Thermal Uniformity: __
Throughput: __
Optics: __
Dimensions: __
External Computer: __
Peak Block Ramp Rate: __
Quantity: 1 Kit
Format:
__
Sample Ramp Rate:
__
Temperature Range (Metric):
__
Passive Reference Dye:
__
Content And Storage:
Contains three Optical 96-Well Reaction Plates, preloaded and sealed containing Cy3™ Texas Red™ and Cy5™ fluorescent dye standards.Store at -15 to -25°C. (This product is light sensitive.)Expiration date: 12 months from the date of delivery
For Use With (Equipment):
7500 System
Reaction Speed:
__
Run Time:
__
Thermal Cycling System:
__
Capacity:
__
Precision:
__
Max. Heating Rate (Thermal Block):
__
Weight:
__
Thermal Accuracy:
__
Sensitivity:
__
Reaction Volume Range:
__
Dynamic Range:
__
Calibrated Dye:
Cy™3, Cy™5, Texas Red™
Thermal Uniformity:
__
Throughput:
__
Optics:
__
Dimensions:
__
External Computer:
__
Peak Block Ramp Rate:
__
Quantity:
1 Kit
Format: __
Sample Ramp Rate: __
Temperature Range (Metric): __
Passive Reference Dye: __
Content And Storage: Contains one Background Plate and two spectral calibration plates (FAM™, JOE™, NED™, ROX™, SYBR™ Green, TAMRA™, TET™,and VIC™ dye standards).Store at -15 to 25°C. (This product is light sensitive.)Expiration date: 12 months from the date of delivery
For Use With (Equipment): 7900HT Fast System
Reaction Speed: __
Run Time: __
Thermal Cycling System: __
Capacity: __
Precision: __
Max. Heating Rate (Thermal Block): __
Weight: __
Thermal Accuracy: __
Sensitivity: __
Reaction Volume Range: __
Dynamic Range: __
Calibrated Dye: FAM, JOE, NED, ROX, TAMRA™, TMR (Tetramethylrhodamine), VIC, SYBR™ Green
Thermal Uniformity: __
Throughput: __
Optics: __
Dimensions: __
External Computer: __
Peak Block Ramp Rate: __
Quantity: 1 kit
Format:
__
Sample Ramp Rate:
__
Temperature Range (Metric):
__
Passive Reference Dye:
__
Content And Storage:
Contains one Background Plate and two spectral calibration plates (FAM™, JOE™, NED™, ROX™, SYBR™ Green, TAMRA™, TET™,and VIC™ dye standards).Store at -15 to 25°C. (This product is light sensitive.)Expiration date: 12 months from the date of delivery
For Use With (Equipment):
7900HT Fast System
Reaction Speed:
__
Run Time:
__
Thermal Cycling System:
__
Capacity:
__
Precision:
__
Max. Heating Rate (Thermal Block):
__
Weight:
__
Thermal Accuracy:
__
Sensitivity:
__
Reaction Volume Range:
__
Dynamic Range:
__
Calibrated Dye:
FAM, JOE, NED, ROX, TAMRA™, TMR (Tetramethylrhodamine), VIC, SYBR™ Green
Thermal Uniformity:
__
Throughput:
__
Optics:
__
Dimensions:
__
External Computer:
__
Peak Block Ramp Rate:
__
Quantity:
1 kit
Format: __
Sample Ramp Rate: __
Temperature Range (Metric): __
Passive Reference Dye: __
Content And Storage: __
For Use With (Equipment): __
Reaction Speed: __
Run Time: __
Thermal Cycling System: __
Capacity: __
Precision: __
Max. Heating Rate (Thermal Block): __
Weight: __
Thermal Accuracy: __
Sensitivity: __
Reaction Volume Range: __
Dynamic Range: __
Calibrated Dye: __
Thermal Uniformity: __
Throughput: __
Optics: __
Dimensions: __
External Computer: __
Peak Block Ramp Rate: __
Quantity: __
Format:
__
Sample Ramp Rate:
__
Temperature Range (Metric):
__
Passive Reference Dye:
__
Content And Storage:
__
For Use With (Equipment):
__
Reaction Speed:
__
Run Time:
__
Thermal Cycling System:
__
Capacity:
__
Precision:
__
Max. Heating Rate (Thermal Block):
__
Weight:
__
Thermal Accuracy:
__
Sensitivity:
__
Reaction Volume Range:
__
Dynamic Range:
__
Calibrated Dye:
__
Thermal Uniformity:
__
Throughput:
__
Optics:
__
Dimensions:
__
External Computer:
__
Peak Block Ramp Rate:
__
Quantity:
__