Fractogel® EMD Chelate (M)

Description

• General description: Fractogel® EMD Chelate combines our tentacle structure with iminodiacetic acid as the metal chelating ligand
• Features and Benefits: Fractogel® EMD Chelate (M) enables users to:Efficiently purify histidine-tagged recombinant proteins Coordinate metal ions to non-bonding electron pairs of the amino acid side chains and separate peptides Ensure timely and successful startup and operation Minimize hydrophobic interactions due to the tentacles technology, resulting in superior selectivity and recovery Handle multiple CIP and packing cycles with the chemically and physically robust bead, leading to a longer resin lifetime
• Packaging: 1.10338.0100: Fractogel® EMD Chelate (M) Resin 100ml1.10338.0010: Fractogel® EMD Chelate (M) Resin 10 ml1.10338.0500: Fractogel® EMD Chelate (M) Resin 500 ml1.10338.5000: Fractogel® EMD Chelate (M) Resin 5 L
• Analysis Note: Appearance: Milky tubid suspension, free from impurities (foreigen particles)Microscopic evaluation: Uniform spherical particles, no agglomerates, no finesCerium: ≤ 10 µg/gCopper-(II)-binding capacity: 60 - 100 µmol/mlExtractable matter (water): ≤ 0.05 %Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 barParticle size (d10): 37 - 45 µmParticle size (d50): 48 - 60 µmParticle size (d90): 63 - 77 µmColony forming units (TAMC + TYMC): ≤ 100 CFU/mlEndotoxins: ≤ 1.00 EU/mlFunctional test: passes testFunctional test: Separation of chymotrypsinogen A and myoglobin
• Legal Information: FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany