Fractogel® EMD DEAE (M)

Description

• General description: Weak anion exchange Fractogel® resin featuring the tentacle technology, for purification of acidic and neutral proteins and peptides from multiple sources, pDNA purification, DNA, RNA, and endotoxin removal, large viruses purification, vaccine purification, blood fractionation, and more
• Features and Benefits: Fractogel® EMD DEAE (M) enables:Excellent binding to large viruses and plasmid DNAHomogenous binding with high selectivity and purityLower elution volumes for the highest purity levelsCompatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).The strength of the binding depends on the following:the buffer systempH value of the buffer which determines the surface charge of the proteinthe degree of the ionization of the functional groups of the exchangerthe concentration of the counter ionsthe charge density on the support (protein binding capacity)
• Packaging: 1.16883.0100: Fractogel® EMD DEAE (M) Resin 100ml1.16883.0010: Fractogel® EMD DEAE (M) Resin 10ml1.16883.0500: Fractogel® EMD DEAE (M) Resin 500ml1.16883.5000: Fractogel® EMD DEAE (M) Resin 5L
• Analysis Note: Appearance: Milky turbid suspension,free from impurities (foreign particles)Microscopic evaluation: Uniform spherical particles,no agglomerates,no finesExtractable matter (water): ≤ 0.03 %Cerium: ≤ 1 µg/gPressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1 barParticle size (d10): 37 - 45 µmParticle size (d50): 48 - 60 µmParticle size (d90): 63 - 77 µmColony forming units (TAMC + TYMC): ≤ 100 CFU/mlEndotoxins: ≤ 1.00 EU/mlProtein binding capacity (bovine serum albumin): 80 - 120 mg/mlFunctional test (b:a): ≤ 0.25Functional test: Separation of conalbumin and human serum albumin
• Legal Information: FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany