Fractogel® EMD SO3 ⁻ (M) Resin

Description

• General description: Strong cation exchange Fractogel® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides
• Features and Benefits: Fractogel® EMD SO3- (M) enables:Increased binding strength at low pHHigh aggregate removal efficiency combined with high dynamic binding capacity Lower elution volumes for the highest purity levelsCompatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solutionDue to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).The strength of the binding depends on the following:the buffer systempH value of the buffer which determines the surface charge of the proteinthe concentration of the counter ionsthe charge density on the support (protein binding capacity)
• Packaging: 1.16882.0100: Fractogel® EMD SO3- (M) Resin 100ml1.16882.0010: Fractogel® EMD SO3- (M) Resin 10ml1.16880.0500: Fractogel® EMD SO3- (M) Resin 500ml1.16882.5000: Fractogel® EMD SO3- (M) Resin 5L
• Analysis Note: Appearance: Milky turbid suspension,free from impurities(foreign particles)Microscopic evaluation: Uniform spherical particles,no agglomerates, no finesExtractable matter (water): ≤ 0.05 %Cerium: ≤ 1 µg/gPressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 barParticle size (d10): 37 - 45 µmParticle size (d50): 48 - 60 µmParticle size (d90): 63 - 77 µmColony forming units (TAMC + TYMC): ≤ 100 CFU/mlEndotoxins: ≤ 1.00 EU/mlProtein binding capacity (lysozyme): 110 - 150 mg/mlFunctional test (b:a): ≤ 0.4Functional test (c:d): ≤ 0.45Functional test: Separation chymotrypsinogen A, cytochrom C and lysozyme
• Legal Information: FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany