Thermo Scientific™ Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse

Description

• Thermo Scientific™ Pierce Rapid Mouse Antibody Isotyping Kits are single-use, cassette-based kits for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity
• The kits are composed of small cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well
• The kits determine mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM
• The assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette
• Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample
• These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane
• Results are displayed as a red band indicating the antibody isotype or subclass
• Highlights: Long shelf life – stable for more than 1 year at room temperature Single-step – add diluted antibody sample to the loading-well of cassette Fast – within 5 minutes a band appears that indicates the antibody isotype Sensitive – detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10ng/mL Specific – assay does not crossreact with fetal bovine serum (FBS) Reliable – results are similar to the standard ELISA-based isotyping assays Includes: Ten pouches, each containing two (Part No
• 26178) or three (Part No
• 26179) cassettes, plus a bottle of sample diluent