PI23325
Thermo Scientific™ Easy-Titer™ Human IgG (gamma chain) Assay Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI23325-Each-of-1 | In Stock | ₹ 63,271.62 |
PI23325 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 63,271.62
GST (18%): ₹ 11,388.892
Total Price: ₹ 74,660.512
Description
Easy-Titer Human IgG (Gamma Chain) Assay Kit
Target Specificity
Target-specific
For Use With (Equipment)
Spectrophotometer, Microplate Reader
Assay
Protein Quantitation Assay, Isotyping Assay, Immunoassay
Content And Storage
Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Human IgG (gamma chain) Sensitized Polystyrene Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).
Quantity
96 tests
For Use With (Application)
Solution-based Detection, Absorbance
Product Line
Easy-Titer™
Detection Method
Colorimetric
Related Products
Description
- Thermo Scientific™ Easy-Titer Antibody Assay Kits enable accurate determination of antibody concentration in serum and culture supernatant, and include kits specific for mouse IgG, rabbit IgG, human IgG (H+L), human IgG (Fc), or human IgM
- These kits include antibody-sensitized microspheres to measure the specific concentration of mouse, rabbit and human antibodies by an easy and rapid micro-agglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer)
- Each kit is specific for a particular species and class of immunoglobulin and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins
- The kits are extremely sensitive, requiring very small sample volumes
- Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately)
- Highlights: Kits for five popular targets – choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody Antibody-based specificity – measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration Faster and easier than ELISA – three-component, homogenous assay; 10 minutes total incubation time No special equipment needed – uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm) Sensitive – assay range (standard curve) 8 to 500ng/mL; requires starting sample of at least 300ng/mL Reproducible – coefficient of variation less than 5%; error depends on dilution and pipetting technique Antibody standards sold separately – use any antibody standard with proper target identity and known concentration (greater than 10μg/mL) Easy-Titer Antibody Assays are faster and easier than ELISA: 1
- Prepare standards (5 to 500ng/mL) by diluting purified antibody in Kit Dilution Buffer 2
- Prepare samples by diluting at least 20-fold in Dilution Buffer to within assay range (8 to 500ng/mL)
- 3
- Vortex vial of microsphere beads to create homogeneous suspension
- 4
- Pipette 20μL of bead suspension and 20μL of each sample and standard into 96-well microplate wells
- 5
- Incubate microplate for 5 minutes with vigorous mixing
- 6
- Add 100μL of Kit Blocking Reagent
- 7
- Incubate microplate for 5 minutes with vigorous mixing
- 8
- Measure absorbance on standard plate reader (340nm or 405nm)
- 9
- Plot standard curve and interpolate samples to determine concentration
- Includes: Each kit contains target-specific IgG sensitized microspheres, dilution buffer and blocking buffer
Compare Similar Items
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Description: Easy-Titer Human IgG (Gamma Chain) Assay Kit
Target Specificity: Target-specific
For Use With (Equipment): Spectrophotometer, Microplate Reader
Assay: Protein Quantitation Assay, Isotyping Assay, Immunoassay
Content And Storage: Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Human IgG (gamma chain) Sensitized Polystyrene Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).
Quantity: 96 tests
For Use With (Application): Solution-based Detection, Absorbance
Product Line: Easy-Titer™
Detection Method: Colorimetric
Description:
Easy-Titer Human IgG (Gamma Chain) Assay Kit
Target Specificity:
Target-specific
For Use With (Equipment):
Spectrophotometer, Microplate Reader
Assay:
Protein Quantitation Assay, Isotyping Assay, Immunoassay
Content And Storage:
Sufficient For: 96 total tests (including 9 standards per use)• Goat Anti-Human IgG (gamma chain) Sensitized Polystyrene Beads, 2mL• Easy-Titer Dilution Buffer, 30 mL• Easy-Titer Blocking Buffer, 15 mLStore at 4°C (do not freeze).
Quantity:
96 tests
For Use With (Application):
Solution-based Detection, Absorbance
Product Line:
Easy-Titer™
Detection Method:
Colorimetric
Description: Controlled Protein-Protein Crosslinking Kit
Target Specificity: __
For Use With (Equipment): __
Assay: __
Content And Storage: Sufficient For: One conjugation with two purified proteins (up to 5 mg each)• Sulfo-SMCC Crosslinking Agent, 2 mg• Activation/Conjugation Buffer (10X), 20 mL• PBS packs (each makes 500mL), 2 packs• 2-Mercaptoethylamine-HCl, 6 mg• Immobilized-Reductant, 0.2 mL• SATA, 2 mg• Dimethylformamide (DMF), 1 mL• Hydroxylamine-HCl, 5 mg• Dextran Desalting Column, 2 x 10 mL• Column Extender, 2 ea.• Cysteine-HCl, 20 mg• Ellman's Reagent, 2 mgStore at 4°C.
Quantity: 1 Reaction
For Use With (Application): __
Product Line: Pierce™
Detection Method: __
Description:
Controlled Protein-Protein Crosslinking Kit
Target Specificity:
__
For Use With (Equipment):
__
Assay:
__
Content And Storage:
Sufficient For: One conjugation with two purified proteins (up to 5 mg each)• Sulfo-SMCC Crosslinking Agent, 2 mg• Activation/Conjugation Buffer (10X), 20 mL• PBS packs (each makes 500mL), 2 packs• 2-Mercaptoethylamine-HCl, 6 mg• Immobilized-Reductant, 0.2 mL• SATA, 2 mg• Dimethylformamide (DMF), 1 mL• Hydroxylamine-HCl, 5 mg• Dextran Desalting Column, 2 x 10 mL• Column Extender, 2 ea.• Cysteine-HCl, 20 mg• Ellman's Reagent, 2 mgStore at 4°C.
Quantity:
1 Reaction
For Use With (Application):
__
Product Line:
Pierce™
Detection Method:
__
Description: __
Target Specificity: __
For Use With (Equipment): __
Assay: __
Content And Storage: Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein• SATA, 2 mg• Hydroxylamine·HCl, 5 mg• Conjugation Buffer Stock (10X), 20 mL• PBS pack (makes 500 mL), 1 pack• Dimethylformamide (DMF), 1 mL• Dextran Desalting Column, 1 x 5 mL• Column Extender, 1• Ellman's Reagent (DTNB), 2 mg• Cysteine·HCl, 20 mgStore at 4°C.
Quantity: 1 Reaction
For Use With (Application): __
Product Line: Pierce™
Detection Method: __
Description:
__
Target Specificity:
__
For Use With (Equipment):
__
Assay:
__
Content And Storage:
Sufficient For: One modification experiment with 0.5 to 5 mg of purified protein• SATA, 2 mg• Hydroxylamine·HCl, 5 mg• Conjugation Buffer Stock (10X), 20 mL• PBS pack (makes 500 mL), 1 pack• Dimethylformamide (DMF), 1 mL• Dextran Desalting Column, 1 x 5 mL• Column Extender, 1• Ellman's Reagent (DTNB), 2 mg• Cysteine·HCl, 20 mgStore at 4°C.
Quantity:
1 Reaction
For Use With (Application):
__
Product Line:
Pierce™
Detection Method:
__
Description: __
Target Specificity: __
For Use With (Equipment): __
Assay: __
Content And Storage: Sufficient For: 10 mini gel or blot experiments with a biotinylated-protein probe• Streptavidin-HRP Conjugate, 0.1 mg• Dilution Buffer (10X), 50 mL• PBS Packs (each makes 500 mL), 17 packs• Luminol Enhancer, 55 mL• Stable Peroxide Buffer, 55 mL• Surfact-Amps 20 Detergent Solution, 6 x 10 mL• Pre-Cut Cellophane, 10 sheetsStore kit at 4°C.
Quantity: 220 mL
For Use With (Application): __
Product Line: Pierce™
Detection Method: Chemiluminescence
Description:
__
Target Specificity:
__
For Use With (Equipment):
__
Assay:
__
Content And Storage:
Sufficient For: 10 mini gel or blot experiments with a biotinylated-protein probe• Streptavidin-HRP Conjugate, 0.1 mg• Dilution Buffer (10X), 50 mL• PBS Packs (each makes 500 mL), 17 packs• Luminol Enhancer, 55 mL• Stable Peroxide Buffer, 55 mL• Surfact-Amps 20 Detergent Solution, 6 x 10 mL• Pre-Cut Cellophane, 10 sheetsStore kit at 4°C.
Quantity:
220 mL
For Use With (Application):
__
Product Line:
Pierce™
Detection Method:
Chemiluminescence