PI44916
Thermo Scientific™ Pierce™ Thiophilic Adsorption Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44916-Each-of-1 | In Stock | ₹ 74,226.00 |
PI44916 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 74,226.00
GST (18%): ₹ 13,360.68
Total Price: ₹ 87,586.68
Product Type
Adsorption Kit
Column Type
Agarose Resin, Affinity
Stationary Phase
Sulfone Thioether
Description
Pierce Thiophilic Adsorption Kit
Quantity
4 Columns
Product Line
Pierce™
Format
Gravity Flow Column
Purification Target
IgG Antibody
Content And Storage
Sufficient For: Purifying up to 60 mg immunoglobulin per column• Thiophilic Adsorbent Columns, 3 mL, 4 columns• Binding Buffer, 1 L• Elution Buffer, 1 L• Column Storage Buffer (2X), 100 mL• Guanidine-HCl Crystals, 230 g• Column AccessoriesStore at 4°C.
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Description
- Thermo Scientific™ Pierce Thiophilic Adsorbent is a non-proteinaceous immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human
- Pierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups
- The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.5M potassium sulfate
- In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples
- Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH
- Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions
- The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity
- The resin is available as both a standalone product and as a purification kit containing pre-packed columns and ready-to-use buffers
- Highlights: High capacity – gently bind and purify approx
- 20mg polyclonal antibody per milliliter of adsorbent resin Broad antibody specificity – effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY Simple and fast – quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps Gentle – binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibody Includes: Kit contains four Thiophilic Adsorbent Columns (3mL each), ready-to-use binding and elution buffers, column regenerant and storage buffer, and column accessories Recommended for: Mild isolation of immunoglobulins from human serum; Rapid purification of mouse monoclonals from the culture media or ascites fluid; Selective removal of immunoglobulins from fetal calf serum - useful for cell culture in monoclonal antibody production; Rapid, straightforward procedure yielding essentially pure immunoglobulins from crude rabbit serum; Purification of IgY from chicken; Large-scale purification for biotechnology applications
Compare Similar Items
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Product Type: Adsorption Kit
Column Type: Agarose Resin, Affinity
Stationary Phase: Sulfone Thioether
Description: Pierce Thiophilic Adsorption Kit
Quantity: 4 Columns
Product Line: Pierce™
Format: Gravity Flow Column
Purification Target: IgG Antibody
Content And Storage: Sufficient For: Purifying up to 60 mg immunoglobulin per column• Thiophilic Adsorbent Columns, 3 mL, 4 columns• Binding Buffer, 1 L• Elution Buffer, 1 L• Column Storage Buffer (2X), 100 mL• Guanidine-HCl Crystals, 230 g• Column AccessoriesStore at 4°C.
Product Type:
Adsorption Kit
Column Type:
Agarose Resin, Affinity
Stationary Phase:
Sulfone Thioether
Description:
Pierce Thiophilic Adsorption Kit
Quantity:
4 Columns
Product Line:
Pierce™
Format:
Gravity Flow Column
Purification Target:
IgG Antibody
Content And Storage:
Sufficient For: Purifying up to 60 mg immunoglobulin per column• Thiophilic Adsorbent Columns, 3 mL, 4 columns• Binding Buffer, 1 L• Elution Buffer, 1 L• Column Storage Buffer (2X), 100 mL• Guanidine-HCl Crystals, 230 g• Column AccessoriesStore at 4°C.
Product Type: Conjugate Purification Kit
Column Type: __
Stationary Phase: __
Description: __
Quantity: 1 kit
Product Line: Pierce™
Format: __
Purification Target: __
Content And Storage: Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibodyIDA Agarose Column, 2 mL, 1 columnPolyacrylamide Desalting Column, 10 mL, 1 columnColumn Activator Buffer, 25 mLElution Buffer, 35 mLTBS Packs (each makes 500 mL), 2 packsPBS Pack (makes 500 mL), 1 packActivity Preservation Solution, 1 mLStore kit at 4°C.
Product Type:
Conjugate Purification Kit
Column Type:
__
Stationary Phase:
__
Description:
__
Quantity:
1 kit
Product Line:
Pierce™
Format:
__
Purification Target:
__
Content And Storage:
Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibodyIDA Agarose Column, 2 mL, 1 columnPolyacrylamide Desalting Column, 10 mL, 1 columnColumn Activator Buffer, 25 mLElution Buffer, 35 mLTBS Packs (each makes 500 mL), 2 packsPBS Pack (makes 500 mL), 1 packActivity Preservation Solution, 1 mLStore kit at 4°C.
Product Type: __
Column Type: __
Stationary Phase: __
Description: __
Quantity: 2.5 mL
Product Line: Pierce™
Format: __
Purification Target: __
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Type:
__
Column Type:
__
Stationary Phase:
__
Description:
__
Quantity:
2.5 mL
Product Line:
Pierce™
Format:
__
Purification Target:
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Type: __
Column Type: __
Stationary Phase: __
Description: Pierce Fab Preparation Kit
Quantity: 10 Antibody Samples, each containing 0.25 to 4 mg IgG
Product Line: Pierce™
Format: __
Purification Target: __
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Type:
__
Column Type:
__
Stationary Phase:
__
Description:
Pierce Fab Preparation Kit
Quantity:
10 Antibody Samples, each containing 0.25 to 4 mg IgG
Product Line:
Pierce™
Format:
__
Purification Target:
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.