PI44980
Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44980-Each-of-1 | In Stock | ₹ 84,062.70 |
PI44980 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 84,062.70
GST (18%): ₹ 15,131.286
Total Price: ₹ 99,193.986
Quantity
2.5 mL
Product Line
Pierce™
Content And Storage
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
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Description
- Thermo Scientific™ Pierce Mouse IgG 1 ; Fab and F(ab') 2 Preparation Kits use immobilized ficin protease to efficiently digest mouse IgG 1 into Fab or F(ab') 2 fragments, depending on the chosen reaction conditions
- The kits use ficin protease to enzymatically digest the Fc portion of whole IgG to yield Fab or F(ab') 2 fragments
- Because the ficin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free
- An optimized procedure and high-performance desalting and Protein A spin columns make these kits easy to use
- Highlights: Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease Optimized for mouse IgG 1 – the kit procedure is specifically optimized for monoclonal mouse IgG 1 antibodies, providing the best possible yield for this antibody subclass High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time
- Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments Includes: Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
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Quantity: 2.5 mL
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity:
2.5 mL
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity: 10 Antibody Samples, each containing 0.25 to 4 mg IgG
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity:
10 Antibody Samples, each containing 0.25 to 4 mg IgG
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity: 10 Antibody Samples Each Containing 0.25 to 4 mg IgG
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C
Quantity:
10 Antibody Samples Each Containing 0.25 to 4 mg IgG
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C
Quantity: 5 Column Kit
Product Line: SulfoLink™
Content And Storage: Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Quantity:
5 Column Kit
Product Line:
SulfoLink™
Content And Storage:
Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.