PI44680
Thermo Scientific™ Pierce™ Mouse IgG1 Fab and F(ab')2 Micro Preparation Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44680-Each-of-1 | In Stock | ₹ 66,351.78 |
PI44680 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 66,351.78
GST (18%): ₹ 11,943.32
Total Price: ₹ 78,295.10
Quantity
10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line
Pierce™
Content And Storage
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
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Description
- Thermo Scientific™ Pierce Mouse IgG 1 ; Fab and F(ab') 2 Preparation Kits use immobilized ficin protease to efficiently digest mouse IgG 1 into Fab or F(ab') 2 fragments, depending on the chosen reaction conditions
- The kits use ficin protease to enzymatically digest the Fc portion of whole IgG to yield Fab or F(ab') 2 fragments
- Because the ficin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free
- An optimized procedure and high-performance desalting and Protein A spin columns make these kits easy to use
- Highlights: Enzyme-free digestion products – immobilized ficin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease Optimized for mouse IgG 1 – the kit procedure is specifically optimized for monoclonal mouse IgG 1 antibodies, providing the best possible yield for this antibody subclass High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time
- Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments Includes: Ficin agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
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Quantity: 10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity:
10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity: 10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Papain Agarose, 0.5 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity:
10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Papain Agarose, 0.5 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity: 10 Antibody Samples, each containing 25 to 250 μg IgG
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Quantity:
10 Antibody Samples, each containing 25 to 250 μg IgG
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Quantity: 5 mL
Product Line: __
Content And Storage: Content: 5 mL of settled resin.Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Quantity:
5 mL
Product Line:
__
Content And Storage:
Content: 5 mL of settled resin.Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.