PI44988
Thermo Scientific™ Pierce™ F(ab')2 Preparation Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44988-Each-of-1 | In Stock | ₹ 48,426.96 |
PI44988 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,426.96
GST (18%): ₹ 8,716.853
Total Price: ₹ 57,143.813
Description
Pierce F(ab')2 Preparation Kit
Product Line
Pierce™
Quantity
10 Antibody Samples Each Containing 0.25 to 4 mg IgG
Content And Storage
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C
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Description
- Thermo Scientific™ Pierce F(ab') 2 Preparation Kits use immobilized pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab') 2 fragments that retain antigen binding activity
- These kits use pepsin, a nonspecific endopeptidase, to enzymatically digest the Fc portion of whole IgG to yield the fragment known as F(ab') 2
- This fragment is composed of a pair of Fab' units connected by two disulfide bonds
- Because the pepsin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free
- An optimized procedure, high-performance desalting and Protein A spin columns make these kits easy to use
- Highlights: Enzyme-free digestion products – immobilized pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease Suitable for human and other species of IgG – the kit procedure is optimized for human, and rabbit, but pepsin-based digestion is effective for other species and subclasses of IgG (Note: for best results with mouse IgG 1 , use Part No
- 44980) High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time
- Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments Includes: Pepsin agarose resin, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
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Description: Pierce F(ab')2 Preparation Kit
Product Line: Pierce™
Quantity: 10 Antibody Samples Each Containing 0.25 to 4 mg IgG
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C
Description:
Pierce F(ab')2 Preparation Kit
Product Line:
Pierce™
Quantity:
10 Antibody Samples Each Containing 0.25 to 4 mg IgG
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C
Description: __
Product Line: SulfoLink™
Quantity: 5 Column Kit
Content And Storage: Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Description:
__
Product Line:
SulfoLink™
Quantity:
5 Column Kit
Content And Storage:
Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Description: __
Product Line: SulfoLink™
Quantity: 5 Column Kit
Content And Storage: Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Column AccessoriesStore at 4°C protected from light.
Description:
__
Product Line:
SulfoLink™
Quantity:
5 Column Kit
Content And Storage:
Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Column AccessoriesStore at 4°C protected from light.
Description: Pierce Protein A Spin Plate for IgG Screening
Product Line: Pierce™
Quantity: 1 Plate
Content And Storage: Upon receipt store at 4°C
Description:
Pierce Protein A Spin Plate for IgG Screening
Product Line:
Pierce™
Quantity:
1 Plate
Content And Storage:
Upon receipt store at 4°C