PI44985
Thermo Scientific™ Pierce™ Fab Preparation Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44985-Each-of-1 | In Stock | ₹ 76,576.20 |
PI44985 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 76,576.20
GST (18%): ₹ 13,783.716
Total Price: ₹ 90,359.916
Description
Pierce Fab Preparation Kit
Quantity
10 Antibody Samples, each containing 0.25 to 4 mg IgG
Product Line
Pierce™
Content And Storage
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Related Products
Description
- Thermo Scientific™ Pierce Fab Preparation Kits use immobilized papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose
- These kits use papain, a nonspecific thiol-endopeptidase, to enzymatically cleave whole IgG just above the hinge region to create two separate Fab fragments and one Fc fragment per antibody molecule
- Because the papain protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free
- The antibody digestion reaction is performed in convenient disposable spin columns that allow efficient removal of the immobilized protease and maximum recovery of the IgG fragments
- Also included in the kits are Protein A spin columns and buffers to efficiently purify the resulting fragments
- Protein A binds the Fc fragments and undigested IgG, allowing the pure Fab fragments to be recovered in the flow-through fraction
- Thermo Scientific Zeba™ Desalting Spin Columns make IgG-sample preparation quick and easy
- Highlights: Enzyme-free digestion products – immobilized papain (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease Suitable for human and other species of Ig G – the kit procedure is optimized for human, mouse and rabbit IgG, but papain-based digestion is effective for many species and subclasses of IgG including rat, goat and pig (Note: for best results with mouse IgG 1 , use Part No
- 44980) High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time
- Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG Provides ready-to-use Fab – digestion and final recovery of purified Fab fragments occurs in neutral pH sodium phosphate buffer, suitable for storage or immediate use in typical applications Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments Includes: Papain agarose resin, cysteine, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
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Description: Pierce Fab Preparation Kit
Quantity: 10 Antibody Samples, each containing 0.25 to 4 mg IgG
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Description:
Pierce Fab Preparation Kit
Quantity:
10 Antibody Samples, each containing 0.25 to 4 mg IgG
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Papain Agarose, 1.25 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Description: Pierce F(ab')2 Preparation Kit
Quantity: 10 Antibody Samples Each Containing 0.25 to 4 mg IgG
Product Line: Pierce™
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C
Description:
Pierce F(ab')2 Preparation Kit
Quantity:
10 Antibody Samples Each Containing 0.25 to 4 mg IgG
Product Line:
Pierce™
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Pepsin Agarose, 1.25 mL• F(ab')2 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C
Description: __
Quantity: 5 Column Kit
Product Line: SulfoLink™
Content And Storage: Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Description:
__
Quantity:
5 Column Kit
Product Line:
SulfoLink™
Content And Storage:
Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Description: __
Quantity: 5 Column Kit
Product Line: SulfoLink™
Content And Storage: Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Column AccessoriesStore at 4°C protected from light.
Description:
__
Quantity:
5 Column Kit
Product Line:
SulfoLink™
Content And Storage:
Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Column AccessoriesStore at 4°C protected from light.