PI44600
Thermo Scientific™ Pierce™ Antibody Clean-up Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44600-Each-of-1 | In Stock | ₹ 38,330.88 |
PI44600 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,330.88
GST (18%): ₹ 6,899.558
Total Price: ₹ 45,230.438
Product Type
Antibody Clean-up Kit
Column Type
Proprietary Resin, Affinity
Stationary Phase
Proprietary Ligand
Content And Storage
Sufficient For: Purifying 10 x 0.5 mL IgG samples• Melon Gel IgG Purification Support, 1.2 mL• Melon Gel Purification Buffer, 100 mL• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Product Line
Pierce™
Format
Kit, Microcentrifuge Spin Column
Purification Target
IgG Antibodies
Quantity
10 Reactions
Related Products
Description
- Thermo Scientific™ Pierce Antibody Clean-Up Kit is for removing BSA, gelatin and other stabilizer proteins from antibody stock solutions before labeling and conjugation procedures
- This is a complete and easy-to-use kit for removing BSA and gelatin stabilizing proteins (up to 1%) from IgG samples
- The protocol uses Thermo Scientific Melon™ Gel to bind these frequently used storage proteins, allowing the antibody to flow through in a purified form
- The antibody is recovered in an amine-free and carboxyl-free buffer, ready for use in downstream applications such as biotinylation, fluorescent labeling, crosslinking, immobilization and enzymatic fragmentation (e.g., Fab preparation)
- Bovine serum albumin (BSA) and gelatin are often added to purified antibody solutions as stabilizers for long-term storage
- Inclusion of 0.2 to 1% (2 to 10mg/mL) BSA or gelatin helps stabilize dilute antibody solutions
- Generally these added proteins do not interfere with immunodetection methods; however, their presence in solution does interfere with amine-reactive conjugation chemistries that are the basis for most biotinylation, fluorescent dye labeling, antibody fragmentation and covalent immobilization methods
- For such methods, the antibody must be isolated from the stabilizing proteins, and the Antibody Clean-up Kit accomplishes this efficiently
- Highlights: Fast – removes carrier protein from antibody solutions in half the time required for purifying antibodies through Protein A or G purification procedures Gentle – no need to neutralize or desalt to mitigate the effects of harsh elution buffers Complete – supplied desalting columns provide for easy buffer exchange into the required purification buffer or quick exchange into final buffer of choice Economical – sufficient reagents to perform 10 antibody clean-up reactions Includes: Melon Gel IgG Purification Support and Buffer, desalting columns, microcentrifuge spin columns and tubes
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Product Type: Antibody Clean-up Kit
Column Type: Proprietary Resin, Affinity
Stationary Phase: Proprietary Ligand
Content And Storage: Sufficient For: Purifying 10 x 0.5 mL IgG samples• Melon Gel IgG Purification Support, 1.2 mL• Melon Gel Purification Buffer, 100 mL• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Product Line: Pierce™
Format: Kit, Microcentrifuge Spin Column
Purification Target: IgG Antibodies
Quantity: 10 Reactions
Product Type:
Antibody Clean-up Kit
Column Type:
Proprietary Resin, Affinity
Stationary Phase:
Proprietary Ligand
Content And Storage:
Sufficient For: Purifying 10 x 0.5 mL IgG samples• Melon Gel IgG Purification Support, 1.2 mL• Melon Gel Purification Buffer, 100 mL• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Product Line:
Pierce™
Format:
Kit, Microcentrifuge Spin Column
Purification Target:
IgG Antibodies
Quantity:
10 Reactions
Product Type: IgG Purification Kit
Column Type: Agarose Resin, Affinity
Stationary Phase: Protein A
Content And Storage: Sufficient For: Purifying 6 to 8 mg mouse IgG per column• Protein A Columns, 1 mL, 5 columns• Protein A IgG Binding Buffer, 1 L• IgG Elution Buffer, 500 mL• Excellulose Desalting Columns, 5 mL, 5 columnsStore at 4°C.
Product Line: Pierce™
Format: Kit, 15 mL Conical Spin Column
Purification Target: IgG Antibodies
Quantity: 5 Column Kit
Product Type:
IgG Purification Kit
Column Type:
Agarose Resin, Affinity
Stationary Phase:
Protein A
Content And Storage:
Sufficient For: Purifying 6 to 8 mg mouse IgG per column• Protein A Columns, 1 mL, 5 columns• Protein A IgG Binding Buffer, 1 L• IgG Elution Buffer, 500 mL• Excellulose Desalting Columns, 5 mL, 5 columnsStore at 4°C.
Product Line:
Pierce™
Format:
Kit, 15 mL Conical Spin Column
Purification Target:
IgG Antibodies
Quantity:
5 Column Kit
Product Type: __
Column Type: __
Stationary Phase: __
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Line: Pierce™
Format: __
Purification Target: __
Quantity: 10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Type:
__
Column Type:
__
Stationary Phase:
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Ficin Agarose, 0.8 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• Protein A Binding Buffer, 50 mL• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Line:
Pierce™
Format:
__
Purification Target:
__
Quantity:
10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Type: __
Column Type: __
Stationary Phase: __
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Papain Agarose, 0.5 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Line: Pierce™
Format: __
Purification Target: __
Quantity: 10 Antibody Samples Each Containing 25 to 250 μg IgG
Product Type:
__
Column Type:
__
Stationary Phase:
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Papain Agarose, 0.5 mL• Cysteine-HCl, 1 g• Fab Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Desalt Spin Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4–8°C.
Product Line:
Pierce™
Format:
__
Purification Target:
__
Quantity:
10 Antibody Samples Each Containing 25 to 250 μg IgG