PI44688
Thermo Scientific™ Pierce™ F(ab')2 Micro Preparation Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44688-Each-of-1 | In Stock | ₹ 58,095.24 |
PI44688 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 58,095.24
GST (18%): ₹ 10,457.143
Total Price: ₹ 68,552.383
Description
Pierce F(ab')2 Micro Preparation Kit
Product Line
Pierce™
Target Gene
ERK5/BMK1 (pan)
Quantity
10 Antibody Samples, each containing 25 to 250 μg IgG
Content And Storage
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Related Products
Description
- Thermo Scientific™ Pierce F(ab') 2 Preparation Kits use immobilized pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab') 2 fragments that retain antigen binding activity
- These kits use pepsin, a nonspecific endopeptidase, to enzymatically digest the Fc portion of whole IgG to yield the fragment known as F(ab') 2
- This fragment is composed of a pair of Fab' units connected by two disulfide bonds
- Because the pepsin protease is supplied in immobilized form as beaded agarose resin, the digestion reaction is easily stopped by removing the resin from the IgG solution; the result is digest products that are enzyme-free
- An optimized procedure, high-performance desalting and Protein A spin columns make these kits easy to use
- Highlights: Enzyme-free digestion products – immobilized pepsin (beaded agarose resin) provides for control of the digestion reaction and complete removal of resulting antibody fragments from the protease Suitable for human and other species of IgG – the kit procedure is optimized for human, rabbit and mouse IgG, but pepsin-based digestion is effective for other species and subclasses of IgG (Note: for best results with mouse IgG 1 , use Part No
- 44980) High capacity – kit provides for digestion and purification from as much as 4mg of IgG at a time
- Use the standard size kit for fragmenting 0.25 to 4mg IgG, and the micro kit for 25 to 250μg IgG Complete and easy to use – kits include all reagents, spin columns and accessories needed to prepare and purify antibody fragments Includes: Pepsin agarose resin, digestion buffer, Protein A columns with binding and elution buffers, spin desalting columns and accessories
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Description: Pierce F(ab')2 Micro Preparation Kit
Product Line: Pierce™
Target Gene: ERK5/BMK1 (pan)
Quantity: 10 Antibody Samples, each containing 25 to 250 μg IgG
Content And Storage: Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Description:
Pierce F(ab')2 Micro Preparation Kit
Product Line:
Pierce™
Target Gene:
ERK5/BMK1 (pan)
Quantity:
10 Antibody Samples, each containing 25 to 250 μg IgG
Content And Storage:
Sufficient For: 10 antibody samples, each containing 25 to 250 μg IgG• Pierce Pepsin Agarose, 0.5 mL• F(ab')2 Digestion Buffer, 55 mL• NAb Protein A Plus Spin Columns, 0.2 mL, 2 columns• PBS Packs (each makes 500 mL), 2 packs• IgG Elution Buffer, 50 mL• Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge Tubes, 2 mL, 30 tubesStore at 4°C
Description: __
Product Line: __
Target Gene: __
Quantity: 5 mL
Content And Storage: Content: 5 mL of settled resin.Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Description:
__
Product Line:
__
Target Gene:
__
Quantity:
5 mL
Content And Storage:
Content: 5 mL of settled resin.Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Description: __
Product Line: Pierce™
Target Gene: __
Quantity: 5 g
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 25°C.
Description:
__
Product Line:
Pierce™
Target Gene:
__
Quantity:
5 g
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 25°C.
Description: AminoLink Immobilization Kit, 2mL
Product Line: AminoLink™
Target Gene: __
Quantity: 5 Column Kit
Content And Storage: Sufficient For: Immobilizing 2 to 20 mg of protein per column• AminoLink Columns, 2mL, 5 columns• AminoLink Coupling Buffer (pH 7.0), 250 mL• Quenching Buffer, 50 mL• Wash Buffer, 240 mL• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Column AccessoriesStore at 4°C
Description:
AminoLink Immobilization Kit, 2mL
Product Line:
AminoLink™
Target Gene:
__
Quantity:
5 Column Kit
Content And Storage:
Sufficient For: Immobilizing 2 to 20 mg of protein per column• AminoLink Columns, 2mL, 5 columns• AminoLink Coupling Buffer (pH 7.0), 250 mL• Quenching Buffer, 50 mL• Wash Buffer, 240 mL• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Column AccessoriesStore at 4°C