PI77667

Thermo Scientific™ Imject™ Maleimide-Activated BSA Spin Kit

Manufacturer: Thermo Scientific™

Select a Size

Pack Size SKU Availability Price
Each of 1 PI77667-Each-of-1 In Stock ₹ 68,105.76

PI77667 - Each of 1

₹ 68,105.76

In Stock

Quantity

1

Base Price: ₹ 68,105.76

GST (18%): ₹ 12,259.037

Total Price: ₹ 80,364.797

Product Line

Imject™

Quantity

5 x 2 mg Kit

Format

Kit

Description

Imject Maleimide-Activated BSA Spin Kit

Content And Storage

Sufficient For: 5 conjugation reactions and complete immunogen preparations, each with up to 2 mg sulfhydryl-containing peptide• Imject Maleimide-Activated BSA, 5 x 2 mg• Imject Maleimide Conjugation Buffer, 30 mL• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore the Maleimide-Activated blue Carrier Protein at -20°C. Store remaining components at 4°C.

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Description

  • Thermo Scientific™ Imject Maleimide-Activated BSA is sulfhydryl-reactive bovine serum albumin for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens
  • Purified, carrier-grade BSA is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer
  • Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens
  • Several antigen molecules can be conjugated to each BSA molecule to increase their immunogenicity and likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest
  • Select from among several package sizes of activated BSA protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns
  • Highlights: Convenient packages – choose stabilized, lyophilized Maleimide-Activated BSA or complete immunogen conjugation kit Optimized activation – specifications require that each lot is activated with 15 to 20 moles of sulhydryl-reactive maleimide per mole of BSA, ensuring consistently high levels of conjugation Sulfhydryl-reactive – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals High-quality carrier protein – pure BSA (Fraction V) is activated using high-quality Thermo Scientific Sulfo-SMCC crosslinker and formulated to ensure high stability and consistent performance Immunogenic and soluble – moderately immunogenic and highly soluble, BSA is useful for preparation of the primary immunogen or as an irrelevant hapten-carrier for antibody screening and immunoassays after using KLH as the carrier protein to generate the immune response against the hapten Includes: Kit contains stabilized, lyophilized Maleimide-Activated BSA (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization

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Description:
Imject Maleimide-Activated BSA Spin Kit

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