PI77670

Thermo Scientific™ Imject™ EDC Blue Carrier™ Protein Spin Kit

Manufacturer: Thermo Scientific™

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Pack Size SKU Availability Price
Each of 1 PI77670-Each-of-1 In Stock ₹ 52,747.74

PI77670 - Each of 1

₹ 52,747.74

In Stock

Quantity

1

Base Price: ₹ 52,747.74

GST (18%): ₹ 9,494.593

Total Price: ₹ 62,242.333

Content And Storage

Sufficient For: 5 EDC conjugation reactions and complete immunogen preparations, each with up to 2 mg peptide• Imject Blue Carrier Protein (in MES buffer), 5 x 2 mg• Imject EDC Conjugation Buffer, 30 mL• EDC Crosslinker, 5 x 10 mg• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore at 4°C

Format

Kit

Product Line

Imject™

Description

Imject EDC Blue Carrier Protein Spin Kit

Quantity

5 x 2 mg Kit

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Description

  • Thermo Scientific™ Blue Carrier Protein is a highly-soluble, mollusk-derived hemocyanin formulated in convenient package sizes and a complete kit for carbodiimide (EDC-mediated) hapten-carrier conjugation via amine and carboxyl groups
  • Blue Carrier Protein is a purified preparation of Concholepas concholepas hemocyanin (CCH)
  • The large protein exhibits most of the same immunogenic properties as the popular carrier protein, keyhole limpet hemocyanin (KLH)
  • However, its signficantly better solubility provides greater flexibility in immunogen preparation protocols by allowing a broader range of buffer and pH conditions for coupling peptides, proteins and other haptens using classical crosslinkers such as glutaraldehyde, Sulfo-SMCC and EDC
  • Highlights: Convenient packages – choose lyophilized Blue Carrier Protein or complete EDC conjugation kit High-yield conjugation – each molecule of CCH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers Validated quality – the immunogenic carrier protein is specially purified to ensure uniform chemical structure and solubility for consistent performance Superior solubility – increased solubility compared to KLH allows Blue Carrier Protein to be used in a broader range of conjugation protocols to accommodate unusual or poorly soluble haptens Highly immunogenic – provides essentially the same level of immunogenicity and phylogenetic distance as keyhole limpet hemocyanin (KLH) Properties of Blue Carrier Protein: Alternative names: Concholepas concholepas hemocyanin, CCH Molecular weight: Whole molecule: ∽8,000,000; Subunits: ∽400,000 and ∽350,000 Absorption: maxima 280nm, 350nm and 558nm Extinction coefficient (Abs
  • 1mg/mL solution): 1.4 at 280nm; 0.35 at 346nm A280/A350 Ratio: ∽4.0 Copper/protein ratio: ∽16 Cu 2+ /protein molecule Isoelectric point: 6.0 Solubility: Soluble in aqueous buffers pH 7.0 to 8.0 Stability: Stable in aqueous buffers (∽pH 7.2 without Ca 2+ or Mg 2+ ) Includes: Kit contains lyophilized Blue Carrier Protein (in MES buffer), EDC crosslinker, buffers and desalting columns to easily prepare ready-to-use immunogens for injection

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Content And Storage:
Sufficient For: 5 EDC conjugation reactions and complete immunogen preparations, each with up to 2 mg peptide• Imject Blue Carrier Protein (in MES buffer), 5 x 2 mg• Imject EDC Conjugation Buffer, 30 mL• EDC Crosslinker, 5 x 10 mg• Imject Purification Buffer Salts, 5 x 0.84 g• Zeba Spin Desalting Columns, 7K MWCO, 2 mL, 5 columnsStore at 4°C

Format:
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Description:
Imject EDC Blue Carrier Protein Spin Kit

Quantity:
5 x 2 mg Kit

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Content And Storage:
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Quantity:
5 x 2 mg

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Content And Storage:
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Content And Storage:
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