Thermo Scientific™ Imject™ Purification Buffer Salts

Description

• Thermo Scientific™ Imject Mariculture Keyhole Limpet Hemocyanin (mcKLH) is formulated to achieve optimum performance with different conjugation chemistries, and includes a complete kit for carbodiimide-mediated hapten-carrier preparation
• Mariculture KLH (mcKLH) is purified and lyophilized in buffers that optimize its stability and solubility for hapten conjugation
• The large, complex protein contains hundreds of sites per molecule for effective conjugation of peptides and other antigens using amine-reactive or carboxyl-reactive crosslinkers
• Preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking
• Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC)
• The Imject EDC Spin Kit includes ready-to-use units of KLH carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method
• Highlights: Convenient packages – choose lyophilized KLH protein or complete EDC conjugation kit High-yield conjugation – each molecule of KLH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers Validated quality – purified and stabilized mariculture KLH maintains solubility in aqueous solutions, unlike traditional sources of the carrier protein Sustainable source – KLH is harvested from select populations of the mollusk Megathura crenulata (keyhole limpet) that are grown in mariculture, not extracted from wild populations Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin