Thermo Scientific™ Imject™ Maleimide Conjugation Buffer

Description

• Thermo Scientific™ Imject Maleimide-Activated mcKLH is sulfhydryl-reactive immunogenic mariculture keyhole limpet hemocyanin (KLH) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens
• The high-quality mariculture KLH is rendered sulfhydryl-reactive by chemical modification with Sulfo-SMCC crosslinker, and the resulting maleimide-activated protein is then stabilized and lyophilized in phosphate buffer
• Maleimide groups form covalent crosslinks with sulfhydryl (-SH) moieties on cysteine residues of peptides and other thiol-containing haptens
• Numerous antigen molecules can be conjugated to each KLH molecule to make them highly immunogenic and increase the likelihood of eliciting immunized animals to produce high-titer antisera to the epitopes of interest
• Select from among several package sizes of activated KLH protein, or choose the complete conjugation kit that includes optimized buffers and trouble-free spin desalting columns
• Highlights: Convenient packages – choose stabilized, lyophilized Maleimide-Activated mcKLH or a complete immunogen conjugation kit Optimized activation – specifications require that each lot is activated with more than 400 moles of sulhydryl-reactive maleimide per mole of KLH, ensuring consistently high levels of conjugation Sulfhydryl-reactiv e – engineered for conjugating cysteine-containing peptide antigens to make effective immunogens for high-titer antibody production Simple, rapid procedure – complete the hapten-carrier conjugation reaction in two hours to yield sufficient immunogen for several injections and booster immunizations of host animals High-quality carrier protein – KLH is isolated from the mollusk Megathura crenulata (keyhole limpet); mcKLH is a high-quality form of KLH that is harvested from limpets grown in mariculture Highly immunogenic – KLH has a high molecular mass (0.5 to 13 million daltons; aggregates of 350 and 390kDa subunits) and elicits a stronger immune response than BSA or ovalbumin Excellent solubility – our preparations of mariculture KLH are more soluble than typical forms and we also offer a PEGylated form for even greater hydrophilicity and solubility Includes: Kit contains stabilized, lyophilized Maleimide-Activated mcKLH (in PBS with stabilizer), buffers and desalting columns to easily prepare ready-to-use immunogens for immunization