69-451-4
MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 69-451-4-Each-of-1 | In Stock | ₹ 31,361.82 |
69-451-4 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,361.82
GST (18%): ₹ 5,645.128
Total Price: ₹ 37,006.948
Efficiency
Guaranteed efficiency: >2 x 107cfu/μg
Content And Storage
≤-70°C
Quantity
1 mL
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Description
- BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
- pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
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Efficiency: Guaranteed efficiency: >2 x 107cfu/μg
Content And Storage: ≤-70°C
Quantity: 1 mL
Efficiency:
Guaranteed efficiency: >2 x 107cfu/μg
Content And Storage:
≤-70°C
Quantity:
1 mL
Efficiency: Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage: ≤-70°C
Quantity: 0.4 mL
Efficiency:
Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage:
≤-70°C
Quantity:
0.4 mL
Efficiency: Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage: ≤-70°C
Quantity: 1 mL
Efficiency:
Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage:
≤-70°C
Quantity:
1 mL
Efficiency: Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage: ≤-70°C
Quantity: 0.4 mL
Efficiency:
Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage:
≤-70°C
Quantity:
0.4 mL