7020066
MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
Manufacturer: Fischer Scientific
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Efficiency
Guaranteed efficiency: >2 x 107cfu/μg
Content And Storage
≤-70°C
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Description
- BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
- pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
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Efficiency: Guaranteed efficiency: >2 x 107cfu/μg
Content And Storage: ≤-70°C
Efficiency:
Guaranteed efficiency: >2 x 107cfu/μg
Content And Storage:
≤-70°C
Efficiency: Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage: ≤-70°C
Efficiency:
Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage:
≤-70°C
Efficiency: Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage: ≤-70°C
Efficiency:
Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage:
≤-70°C
Efficiency: Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage: ≤-70°C
Efficiency:
Guaranteed efficiency: >5 x 106cfu/μg
Content And Storage:
≤-70°C