7020079

MilliporeSigma™ Novagen™ Origami™ B Competent Cells

Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.

Manufacturer: Fischer Scientific

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Efficiency

Guaranteed efficiency: >2 x 106cfu/μg

Content And Storage

≤-70°C

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Description

  • Description Origami™ B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21
  • Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™ and Origami hosts in one strain background
  • The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, these strains are recommended for use only with pET plasmids carrying the ampicillin resistance marker bla.

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