7020095

MilliporeSigma™ Novagen™ Origami™ 2 Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Manufacturer: Fischer Scientific

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Efficiency

Guaranteed efficiency: >2 x 106cfu/μg

Content And Storage

≤-70°C

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Description

  • Description Origami™ 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E
  • coli cytoplasm
  • The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline
  • To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding.

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Guaranteed efficiency: >2 x 106cfu/μg

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≤-70°C

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Guaranteed efficiency: >2 x 106cfu/μg

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Efficiency:
Guaranteed efficiency: >2 x 106cfu/μg

Content And Storage:
≤-70°C