MilliporeSigma™ Novagen™ Origami™ B Competent Cell Set

Description

• Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™ and Origami hosts in one strain background
• The trxB and gor mutations are selectable on kanamycin and tetracycline, respectively; therefore, this strain is recommended for use with pET plasmids carrying the ampicillin resistance marker bla
• DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
• Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
• pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.