7020088

MilliporeSigma™ Novagen™ Rosetta-gami™ B (DE3) Competent Cells

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

Manufacturer: Fischer Scientific

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Content And Storage

≤-70°C

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Description

  • Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm
  • These strains are compatible with ampicillin- or spectinomycin-resistant vectors
  • E3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
  • Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

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