Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm
These strains are compatible with ampicillin- or spectinomycin-resistant vectors
E3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.