71-138-3
MilliporeSigma™ Novagen™ Rosetta-gami™ B (DE3) pLacI Competent Cells
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 71-138-3-Each-of-1 | In Stock | ₹ 18,081.39 |
71-138-3 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 18,081.39
GST (18%): ₹ 3,254.65
Total Price: ₹ 21,336.04
Content And Storage
≤-70°C
Quantity
0.4 mL
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Description
- Expression host strains for pETBlue™ and pTriEx™ vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor
- The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer
- The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA
- Note that these strains are not recommended for use with pET, pACYCDuet™, pCDF, pRSF, pCOLADuet™ or pETcoco™ vectors
- Rosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm
- These strains are compatible with ampicillin- or spectinomycin-resistant vectors
- In Rosetta-gami B(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
