71-059-4
MilliporeSigma™ Novagen™ RosettaBlue™ (DE3) Competent Cells
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 71-059-4-Each-of-1 | In Stock | ₹ 30,509.84 |
71-059-4 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 30,509.84
GST (18%): ₹ 5,491.771
Total Price: ₹ 36,001.611
Efficiency
Guaranteed efficiency: >1 x 108cfu/μg
Content And Storage
≤-70°C
Quantity
1 mL
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Description
- RosettaBlue™ host strains are NovaBlue derivatives that combine high transformation efficiency and recA endA lacI q mutations with enhanced expression of eukaryotic proteins that contain codons rarely used in E
- coli
- These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, and GGA on a chloramphenicol-resistant plasmid
- The tRNA genes are driven by their native promoters
- Blue/white screening is not possible with RosettaBlue(DE3) strains due to the presence of the lacZ α-peptide coding sequence in the λDE3 prophage
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
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Efficiency: Guaranteed efficiency: >1 x 108cfu/μg
Content And Storage: ≤-70°C
Quantity: 1 mL
Efficiency:
Guaranteed efficiency: >1 x 108cfu/μg
Content And Storage:
≤-70°C
Quantity:
1 mL
Efficiency: __
Content And Storage: -20°C
Quantity: 50 μg
Efficiency:
__
Content And Storage:
-20°C
Quantity:
50 μg
Efficiency: __
Content And Storage: __
Quantity: 25 mg
Efficiency:
__
Content And Storage:
__
Quantity:
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Efficiency: __
Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Quantity: 100 μg
Efficiency:
__
Content And Storage:
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Quantity:
100 μg