71-405-3

MilliporeSigma™ Novagen™ Rosetta™ 2 Competent Cell Set

Manufacturer: MilliporeSigma™

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Pack Size SKU Availability Price
Each of 1 71-405-3-Each-of-1 In Stock ₹ 31,876.23

71-405-3 - Each of 1

₹ 31,876.23

In Stock

Quantity

1

Base Price: ₹ 31,876.23

GST (18%): ₹ 5,737.721

Total Price: ₹ 37,613.951

Efficiency

Guaranteed efficiency: 2 x 106cfu/μg

Content And Storage

≤-70°C

Quantity

1 Set

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Description

  • Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E
  • coli
  • These strains supply tRNAs for 7 rare codones (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) on a compatible chloramphenicol-resistant plasmid
  • The tRNA genes are driven by their native promoters
  • DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
  • Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
  • pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

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