71-432-3
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 Competent Cell Set
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 71-432-3-Each-of-1 | In Stock | ₹ 33,423.16 |
71-432-3 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,423.16
GST (18%): ₹ 6,016.169
Total Price: ₹ 39,439.329
Efficiency
Guaranteed efficiency: 2 x 106cfu/μg
Content And Storage
≤-70°C
Quantity
1 Set
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Description
- Rosetta-gami 2 host strains combine the advantages of Rosetta 2 and Origami 2 strains to alleviate codon bias and enhance disulfide bond formation in the cytoplasm when heterologoous proteins are expressed in E
- coli
- These trxB/gor mutants are compatible with kanamycin-resistant vectors, and carry the chloramphenicol-resistant pRARE2 plasmid, which supplies seven rare tRNAs
- DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
- Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
- pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
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Efficiency: Guaranteed efficiency: 2 x 106cfu/μg
Content And Storage: ≤-70°C
Quantity: 1 Set
Efficiency:
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