Invitrogen™ BL21 Star™ (DE3)pLysS One Shot™ Chemically CompetentE. coli

Description

• One Shot BL21 Star (DE3)pLysS Chemically Competent E
• coli are cells designed for applications that require high-level expression of non-toxic recombinant proteins from low or high copy number vectors, such as pRSET and pET
• The BL21 (DE3) E
• coli strain is one of the most popular host strains to produce recombinant proteins and is adapted for research and industrial applications (e.g., first biopharmaceutical recombinant human insulin)
• BL21(DE3) is a derivative of the E
• coli B strain that does not contain the lon protease and is also deficient in the outer membrane protease OmpT
• The lack of two key proteases reduces degradation of heterologous proteins expressed in the strains
• Additionally, several other strain features made them especially suited for protein production, namely fast growth in minimal medium, a lower acetate production when grown in high glucose media, and the ability to reach high cell density
• This strain was engineered to carry the λDE3 lysogen that contains T7 RNA polymerase gene under control of the lac UV5 promoter
• IPTG is required to induce expression of the T7 RNA polymerase cascade system that in turn transcribe T7 promoter regulated target gene
• The pLysS CamR plasmid carried by the BL21 Star (DE3) pLysS strain expresses T7 lysozyme, a T7 RNA polymerase inhibitor that prevents leaky expression in uninduced cells
• The p15a origin of pLysS makes this plasmid compatible with pUC or pBR322-derived plasmids.This strain offers enhanced mRNA stability due to a mutation in the RNaseE gene ( rne 131) that reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield
• The BL21 Star (DE3) pLys strain has lower basal expression of heterologous genes than BL21 Star (DE3) strain
• Other trains such as BL21-AI are recommended for toxic protein expression
• One Shot BL21 Star (DE3) pLysS Chemically Competent cells have a transformation efficiency of >1 x 10 8 cfu/μg plasmid DNA
• One Shot BL21 Star (DE3)pLysS Chemically Competent E
• coli offer: • Transformation efficiency of >1 x 10 8 cfu/μg plasmid DNA • Ion and OmpT protease deficiency reduces degradation of recombinant protein • IPTG-inducible lacUV5 promoter controls expression of the T7 RNA polymerase • rne 131 mutation reduces levels of endogenous RNases and mRNA degradation • pLysS (CamR)plasmid for reduced basal expression in uninduced cells • hsd S mutation allows efficient transformation of unmethylated DNA • Fast growth in minimal medium and ability to reach high cell density Easy-to-use One Shot format BL21 Star (DE3) pLysS Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination
• Genotype F - omp T hsd SB (r B - , m B - ) gal dcm rne 131 (DE3) pLysS (CamR) Find the strain and format that you need • We offer other E
• coli strains for protein expression
• • For expression of toxic proteins, consider BL21-AI One Shot Chemically Competent E
• coli (Cat
• No
• C607003)
• • BL21 Star (DE3) and other strains are available in MultiShot formats for high throughput applications
• • We offer a variety of systems for the expression of recombinant proteins in E
• coli , the Champion pET expression system provides the highest level of protein production available in any expression system.