Invitrogen™ One Shot™ BL21(DE3) Chemically CompetentE. coli

Description

• One Shot BL21(DE3) Chemically Competent E
• coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET
• This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase gene under control of the lac UV5 promoter
• Recombinant proteins that are nontoxic to E
• coli are generally expressed at higher levels in BL21(DE3) cells compared to other strains with tighter expression control
• The BL21(DE3) E
• coli strain is one of the most popular host strains to produce recombinant proteins and adapted in research or industrial applications
• It is a derivative of the E
• coli B strain that does not contain the lon protease and is also deficient in the outer membrane protease OmpT
• The lack of two key proteases reduces degradation of heterologous proteins expressed in the strains
• Additionally, several other strain features make them especially suited for protein production, namely fast growth in minimal medium, a lower acetate production when grown in high glucose media, and the ability to reach high cell density. One Shot BL21(DE3) Chemically Competent E
• coli cells can achieve transformation efficiency of >1 x 10 8 cfu/μg of control DNA
• Notes • The basal expression levels of heterologous genes are significantly higher in BL21(DE3), thus strains such as BL21 AI are recommended for toxic protein expression
• • IPTG is required to induce expression of the T7 RNA polymerase cascade system that in turn transcribe T7 promoter regulated target gene
• One Shot BL21(DE3) Chemically Competent E
• coli offer: • Transformation efficiency of >1 x 10 8 cfu/μg control plasmid DNA • Ion and OmpT protease deficiency reduces degradation of recombinant protein • IPTG-inducible lacUV5 promoter controls expression of the T7 RNA polymerase • hsd S mutation allows efficient transformation of unmethylated DNA • Fast growth in minimal medium and ability to reach high cell density Easy-to-use One Shot format BL21(DE3) Chemically Competent E
• coli cells are supplied in the convenient, single-reaction One Shot format
• The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination
• Genotype: F– omp T hsd SB (rB–, mB–) gal dcm (DE3) Find the strain and format that you need • We offer other E
• coli strains for protein expression
• For expression of toxic proteins, consider BL21-AI™ One Shot™ Chemically Competent E
• coli (Cat
• No
• C607003)
• • BL21 Star (DE3) and other strains are available in MultiShot formats for high throughput applications
• • We offer a variety of systems for the expression of recombinant proteins in E
• coli
• , the Champion pET expression system provides the highest level of protein production available in any expression system
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