Thermo Scientific™ BL21(DE3) Competent Cells

Description

• Suitable for the expression of non-toxic heterologous genes The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG BL21(DE3) is an E
• coli B strain and does not contain the lon protease
• It is also deficient in the outer membrane protease OmpT
• The lack of these two key proteases reduces degradation of heterologous proteins expressed in the cells
• High transformation efficiency: >1x107 cfu/μg pUC19 DNA Specifically constructed for high-level expression of recombinant proteins Genetic markers that minimize RNA and protein degradation Convenient two reactions per vial packaging S.O.C
• Outgrowth medium included Applications:  BL21(DE3) Competent Cells are suitable for high-level T7 expression of recombinant proteins Genotype: F– ompT hsdSB (rB–, mB–) gal dcm (DE3) Note: Not suitable for maintenance of expression plasmids
• Use DH10B or DH5α competent cells that do not carry the gene for T7 RNA polymerase for plasmid DNA propagation (Cat
• Nos
• EC0112, EC0113).