3575921
MP Biomedicals™ Trypsin, Porcine Pancreas
Typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
Manufacturer: Fischer Scientific
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For Use With (Application)
Release adherent cells from tissue culture plates for passaging
Content And Storage
4°C
Product Type
Trypsin
Sample Type
Porcine
Form
Powder
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Description
- CAS Number: 9002-07-7 Molecular Weight: Approximately 23,000 or 22000 (for human) Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin
- a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain
- Activity: One BAEE unit will produce a DA253 of 0.001 per minute at pH 7.6 at 25°C using BAEE as substrate
- Reaction volume = 3.2mL (1 cm light path)
- One TAME unit hydrolyzes 1 umole of p-toluene-sulfonyl-L-arginine methyl ester (TAME) per minute at 25°C, pH 8.2, in the presence of 0.001 M calcium ion
- One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified
- Activity Conversion:1 TAME unit = 19.2 USP or NF units = 57.5 BAEE Units Composition: Trypsin is composed of two subunits, a-trypsin and b-trypsin
- a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain.
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