Thermo Scientific™ Trypsin, TPCK Treated

Description

• Thermo Scientific™ Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment
• Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment
• Features of Thermo Scientific Pierce TPCK Trypsin • Cleaves at carboxyl side of arginine and lysine residues • Digestion conditions: pH 7.5–9.0, 37°C Trypsin has a wide range of applications including amino acid analysis and protein sequencing, mapping and structural studies
• Enzymes such as trypsin and chymotrypsin have become important tools in sequencing studies since they are highly selective in their cleavage of peptide bonds
• Trypsin cleaves only those peptide bonds in which the carboxyl group is contributed by a lysine or an arginine residue, regardless of the length or amino acid sequence of the chain
• Our Immobilized TPCK Trypsin can be substituted for free trypsin in many application and is advantageous because it minimizes autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the trypsin
• Immobilized trypsin is also more stable against heat-induced denaturation, resulting in longer maintenance of activity
• Applications: • Removal of adherent cells from tissue culture flasks • Preparing tryptic fragments for Edman degradation sequencing • Immobilized trypsin can be used to purify soybean trypsin inhibitor • Use our Pierce Trypsin, MS Grade (Cat No
• 90057) for mass spectrometry workflows Trypsin is a 23.8kDa pancreatic serine endoprotease derived from trypsinogen, an inactive precursor zymogen, after enzymatic removal of an n-terminal leader sequence by enterokinase
• Once some trypsin has been formed it can catalyze the conversion of more trypsinogen into its catalytically-active form
• Trypsin is treated with L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotrypsin activity without affecting trypsin activity
• Dissolving 0.25 g of trypsin in 100 mL of chilled HBSS for a 0.25% w/v solution is recommended
• Mix solution until homogenous and pH is 7.4 to 7.8
• Filter the solution through a low protein-binding filter such as the Nalgene™ Rapid-Flow™ Sterile Disposable Filter Units with PES Membranes (Cat No
• 567-0010) and store -5°C to -20°C
• Related Products Immobilized Trypsin, TPCK Treated (Agarose Resin) (Cat
• No
• 20230)