PI44881
Thermo Scientific™ Immobilized Ficin (Agarose Resin)
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Pack of 1 | PI44881-Pack-of-1 | In Stock | ₹ 33,375.00 |
PI44881 - Pack of 1
In Stock
Quantity
1
Base Price: ₹ 33,375.00
GST (18%): ₹ 6,007.50
Total Price: ₹ 39,382.50
Content And Storage
Content: 5 mL of settled resin.Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Form
Liquid Suspension
Quantity
5 mL
Product Type
Ficin
Related Products
Description
- Thermo Scientific™ Pierce Immobilized Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilized onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1
- Ficin, which is similar to papain, is a nonspecific, sulfhydryl protease isolated from fig latex
- The molecular weight of Ficin is approximately 25,000 and it is effective at pH 4-9.5 with an optimum pH of 6.5
- When IgG molecules are incubated with ficin in the presence of cysteine, one or more peptide bonds in the hinge region are split, producing either Fab fragments or F(ab')2 fragments
- Mouse IgG1 is notably difficult to digest into functional Fab or F(ab')2 fragments using papain or pepsin, however, yields and immunoreactivity are better when digesting mouse IgG1 with ficin
- Immobilized Ficin was specifically designed for cleavage of mouse IgG1 into or Fab or F(ab')2 fragments, and is advantageous because it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the ficin or controlling the flow of sample over an immobilized ficin column
- Highlights: Can generate both Fab and F(ab')2 fragments from mouse IgG1 Reaction can be easily controlled Antibody fragments are free of autodigestion products Ficin contamination into antibody fragments is eliminated
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Content And Storage: Content: 5 mL of settled resin.Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Form: Liquid Suspension
Quantity: 5 mL
Product Type: Ficin
Content And Storage:
Content: 5 mL of settled resin.Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
Form:
Liquid Suspension
Quantity:
5 mL
Product Type:
Ficin
Content And Storage: Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 25°C.
Form: Solid
Quantity: 5 g
Product Type: Cysteine Hydrochloride
Content And Storage:
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 25°C.
Form:
Solid
Quantity:
5 g
Product Type:
Cysteine Hydrochloride
Content And Storage: Sufficient For: Immobilizing 2 to 20 mg of protein per column• AminoLink Columns, 2mL, 5 columns• AminoLink Coupling Buffer (pH 7.0), 250 mL• Quenching Buffer, 50 mL• Wash Buffer, 240 mL• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Column AccessoriesStore at 4°C
Form: __
Quantity: 5 Column Kit
Product Type: __
Content And Storage:
Sufficient For: Immobilizing 2 to 20 mg of protein per column• AminoLink Columns, 2mL, 5 columns• AminoLink Coupling Buffer (pH 7.0), 250 mL• Quenching Buffer, 50 mL• Wash Buffer, 240 mL• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Column AccessoriesStore at 4°C
Form:
__
Quantity:
5 Column Kit
Product Type:
__
Content And Storage: Store desiccated at room temperature. Shipped at ambient temperature
Form: Powder
Quantity: 2 x 1 g
Product Type: __
Content And Storage:
Store desiccated at room temperature. Shipped at ambient temperature
Form:
Powder
Quantity:
2 x 1 g
Product Type:
__