Thermo Scientific™ WELQut Protease (5 U/μL)

Description

• Thermo Scientific™ WELQut Protease is a highly specific serine protease cleaving outside the WELQX recognition motif to remove N-terminal protein fusion tags
• Thermo Scientific WELQut Protease is highly specific, recombinant serine protease of Staphylococcus aureus
• It recognizes and precisely cleaves recombinant proteins containing an engineered recognition sequence† W- E- L- Q↓X (Trp, Glu, Leu, Gln, X can be any amino acid)
• The protease cleaves outside the recognition sequence without leaving additional amino acids bound to the target protein
• The WELQut Protease is active in a broad temperature (4 to 30°C) and pH (pH 6.5 to 9.0) range and does not require specific buffers
• In addition, this new protease has several procedural advantages - it is ideal for on-column proteolysis reactions and can be easily removed from reaction mixtures using its built-in His-tag
• Highlights Cleaves outside WELQ recognition sequence, without leaving additional amino acids bound to the target protein Highly specific to cognate recognition site, does not generate non-specific product bands, even after long incubation and using excess of protease Easy to remove from the reaction mixture using built-in His-tag Ideal for on-column proteolysis reactions Applications Removal of N-terminal fusion tags from recombinant protein preparations
• Footnote † This cleavage sequence is present in expression vector pLATE52 included into the Thermo Scientific aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WELQut) (#K1281) available from Thermo Scientific
• Definition of Activity Unit: Each unit is defined as the amount of enzyme required to cleave ≥ 99% of 100 μg of a control protein in 16 h at 20°C
• Enzyme activity is assayed in 100 μL 100 mM Tris-HCl (pH 8.0) Hazardous: No Molecular Weight: 22 kDa monomer Source: Bacillus subtilis cells with a cloned gene of SplB protease from Staphylococcus aureus Storage Buffer: Enzyme is supplied in 10 mM Na 2 HPO 4 ; 1.8 mM KH 2 PO 4 , pH 7.3; 140 mM NaCl; 2.7 mM KCl, 50% glycerol Storage Condition:-20°C -20°C