Thermo Scientific™ DyLight™ 650-4xPEG NHS Ester

Description

• Thermo Scientific DyLight 650-4xPEG Amine-Reactive Dye is a derivative of our high-performance DyLight 650 Dye that can be used to fluorescently label antibodies and other proteins for far-red detection
• The DyLight 680-4xPEG dye contains 4 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them
• Conjugates made with DyLight 680-4xPEG Dye can be used as molecular probes for cellular imaging and other fluorescence detection methods
• The far-red to NIR fluorescence properties of DyLight 680-4xPEG Dye make it especially useful in a variety of biological, chemical, and pharmaceutical applications, including in vivo imaging
• The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention
• Highlights: High fluorescence intensity - significantly brighter fluorescence than Alexa Fluor™ 647 PEGylated - improves solubility in aqueous solution and aids in cell permeability Applications: Fluorescence microscopy In vivo or ex vivo imaging Cell-based assays Flow cytometry/fluorescence-activated cell sorting (FACS) Product Details: DyLight 650-4xPEG Amine-Reactive Dye is activated with an N -hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds
• Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled
• However, the high solubility of DyLight Fluors permits protein solutions to be added directly to the labeling reagent.