Thermo Scientific™ DyLight™ 425Q NHS Ester

Description

• Thermo Scientific™ DyLight Quencher Specialty Dyes are for efficient paring with DyLight Fluors and other commercial fluorescent dyes for qualitative and quantitative fluorescence resonance energy transfer (FRET) applications
• DyLight Quencher Specialty Dyes are a family of non-fluorescent dyes designed for fluorescence quenching in FRET applications
• The optimal quencher dye can be selected based upon its characteristic absorption properties that match the emission of a corresponding fluor
• Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation
• Highlights: Large selection – the largest family of dyes available for far red-emitting fluorescence applications NHS ester reactive group –allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation Criteria to consider when choosing a DyLight Quencher Specialty Dye: Absorption wavelength–choose the quencher dye with the absorbance wavelength that matches the emission of your fluorophore and the instrument
• Water solubility–choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure
• More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate
• More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo , meaning that dyes with fewer sulfonates may work best for some applications
• DyLight dye selection–the broad selection of red-emitting dyes allows a number of candidate dyes to be tested in an application for optimal performance
• Recommended for: FRET; Other quenching applications