Thermo Scientific™ GlycoLink™ Coupling Catalyst

Description

• The Thermo Scientific™ GlycoLink Coupling Catalyst decreases reaction times and increases aldehyde-hydrazide coupling efficiency, resulting in greater than 90% coupling of glycoproteins in 4 hours
• The GlycoLink Coupling Catalyst is a two-component kit composed of aniline and sodium acetate buffer
• The catalyst accelerates the coupling rate of hydrazide and alkoxyamine moieties with reactive aldehydes that have been generated by periodate-oxidation of cis-diols in glycoproteins
• The GlycoLink Coupling Catalyst increases the coupling efficiency of these reactions, decreasing the molar excess amounts of labeling reagents that are required for conjugation reactions
• The catalyst is also useful for increasing the binding efficiency of oxidized glycoproteins or other carbohydrate-containing molecules to hydrazide resins
• Highlights: Optimized – catalyzes aldehyde-hydrazide and aldehyde-alkoxyamine conjugation reaction Efficient – maximizes coupling efficiency and decreases required coupling reaction incubation time Gentle – does not interfere with protein function and is easily removed by desalting or dialysis Recommended for: Labeling aldehyde-containing molecules with alkoxyamine-biotins; Labeling aldehyde-containing molecules with hydrazide-biotins; Increase coupling efficiency of aldehyde-containing molecules to hydrazide resin