Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin

Description

• Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent
• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved Very short - spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only.