Thermo Scientific™ Pierce™ TMT11plex Yeast Digest Standard

Description

• The Pierce TMT11plex Yeast Digest Standard consists of four congenic strains of Saccharomyces cerevisiae (a parental line, BY4741, and three lines lacking the non-essential proteins Met6, His4, or Ura2) labeled in triplicate (knock-out strains) or duplicate (parental strain) with TMT11-plex label reagents
• The knock-out strains are used in combination with the parental strain to enable comparison of peptide and protein abundance for quantitation
• Pierce TMT11plex Yeast Digest Standard: • Convenient —ready-to-use lyophilized powder pre-labeled with TMT11plex reagents • Quality —yeast strains verified for genotype, equimolar mixing of the TMT-labeled strains • Quantitative —measure the quantitative precision of TMT reporter ions for 11 channels • Diagnostic —excellent QC assay tool for protein quantitation using TMT on LC-MS/MS instruments Quantitative proteomic strategies using TMT reagents enable multiplexing and precise measurement of protein abundance
• Successful execution of TMT quantitation includes optimized chromatography, mass spectrometry (MS), and data analysis
• A critical parameter for success in this workflow is the ability to consistently detect TMT fragment ions across 11 channels for multiplexing
• Our TMT11plex Yeast Digest standard has been specifically designed to assess LC-MS/MS system performance for TMT quantitation, MS method optimization and system validation
• For method development, TMT11plex Yeast Digest Standard can be used to measure the accuracy, precision, and dynamic range of different mass spectrometry approaches
• These standards can help optimize offline fractionation, chromatography, mass spectrometry acquisition, and data analysis settings
• Multifunctional applications using this standard reduces inter-experiment variability to provides reproducible TMT quantitation
• References: 1
• Paulo JA, O'Connell JD, Gygi SP
• A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments
• J Am Soc Mass Spectrom
• 2016 10:1620-5
• 2
• Yang F, Shen Y, Camp DG 2nd, Smith RD
• High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysis
• Expert Rev Proteomics
• 2012 9(2):129-34
• 3
• Dayon L, Pasquarello C, Hoogland C, Sanchez JC, Scherl A
• Combining low- and high-energy tandem mass spectra for optimized peptide quantification with isobaric tags
• J Proteomics
• 2010 73(4):769-77
• 4
• Diedrich JK, Pinto AF, Yates JR 3rd
• Energy dependence of HCD on peptide fragmentation: stepped collisional energy finds the sweet spot
• J Am Soc Mass Spectrom
• 2013 24(11):1690-9
• 5
• Chiva C, Sabidó E
• HCD-only fragmentation method balances peptide identification and quantitation of TMT-labeled samples in hybrid linear ion trap/orbitrap mass spectrometers
• J Proteomics
• 2014 96:263-70
• Related products: Pierce HeLa Protein Digest Standard (Cat
• No
• 88329) Pierce Peptide Retention Time Calibration Mixture (Cat
• No
• 88321) TMT10plex isobaric label reagent set plus TMT11-131C label reagent (Cat
• No
• A34808)