89-014-357

Abnova ES-E14TG2a (Mouse pluripotent embryonic stem cell) whole cell lysate (nondenatured)

Manufacturer: Abnova Corporation

Select a Size

Pack Size SKU Availability Price
Each of 1 89-014-357-Each-of-1 In Stock ₹ 40,812.12

89-014-357 - Each of 1

₹ 40,812.12

In Stock

Quantity

1

Base Price: ₹ 40,812.12

GST (18%): ₹ 7,346.182

Total Price: ₹ 48,158.302

Tissue

Embryonic

Description

Whole cell lysate (non-denatured)

Preparation Method

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity

50 μg

Host Species

Mouse

Content And Storage

Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application)

Immunoprecipitation, Western Blot

Lysis Buffer

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing

12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer

In modified RIPA Lysis Buffer.

Concentration

4 mg/mL

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Description

  • Maintained in the undifferentiated state using specialized culturing protocols Monitored for undifferentiated cell morphology Analyzed using Flow Cytometry against specific cell marker Coommasie Blue Staining of total cell lysate in SDS-PAGE Western Blotting, Immunoprecipitation

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Abnova Corporation

89-014-357

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Tissue:
Embryonic

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
50 μg

Host Species:
Mouse

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
4 mg/mL

Img

Abnova Corporation

89-014-360

--


Tissue:
Skin

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Human

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
5 mg/mL

Img

Abnova Corporation

89-014-361

--


Tissue:
Placenta

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Human

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
5 mg/mL

Img

Abnova Corporation

89-014-362

--


Tissue:
Prostate

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Human

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Concentration:
5 mg/mL