89-014-397

Sol8 (mouse myoblast) Whole cell lysate, Non-denatured, Abnova

Manufacturer: Abnova Corporation

Select a Size

Pack Size SKU Availability Price
Each of 1 89-014-397-Each-of-1 In Stock ₹ 21,218.88

89-014-397 - Each of 1

₹ 21,218.88

In Stock

Quantity

1

Base Price: ₹ 21,218.88

GST (18%): ₹ 3,819.398

Total Price: ₹ 25,038.278

Tissue

Skeletal Muscle

Description

Whole cell lysate (non-denatured)

Preparation Method

Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity

200 μg

Host Species

Mouse

For Use With (Application)

Immunoprecipitation, Western Blot

Lysis Buffer

Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing

12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer

In modified RIPA Lysis Buffer.

Content And Storage

Store at -80°C. Aliquot to avoid repeated freezing and thawing.

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Description

  • Tissue: Skeletal Muscle Host: Mouse Lysis Buffer: Modified RIPA Lysis Buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF Storage buffer: In modified RIPA Lysis Buffer Western Blotting, Immunoprecipitation

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Abnova Corporation

89-014-397

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Tissue:
Skeletal Muscle

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Mouse

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Img

Abnova Corporation

89-014-398

--


Tissue:
Lung

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Mouse

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Img

Abnova Corporation

89-014-399

--


Tissue:
Melanoma

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Mouse

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Img

Abnova Corporation

89-014-400

--


Tissue:
Bone Marrow

Description:
Whole cell lysate (non-denatured)

Preparation Method:
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay).

Quantity:
200 μg

Host Species:
Mouse

For Use With (Application):
Immunoprecipitation, Western Blot

Lysis Buffer:
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.

Quality Control Testing:
12.5% SDS-PAGE Stained with Coomassie Blue.

Storage Buffer:
In modified RIPA Lysis Buffer.

Content And Storage:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.