Rat spleen tissue lysate, Denatured; Abnova
Manufacturer: Abnova Corporation
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 89-014-514-Each-of-1 | In Stock | ₹ 21,047.76 |
89-014-514 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 21,047.76
GST (18%): ₹ 3,788.597
Total Price: ₹ 24,836.357
For Use With (Application)
Western Blot
Description
Whole tissue lysate (denatured)
Preparation Method
Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Quantity
200 μg
Host Species
Rat
Content And Storage
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue
Spleen
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Concentration
3 mg/mL
Related Products
Description
- Western Blotting
Compare Similar Items
Show Difference
For Use With (Application): Western Blot
Description: Whole tissue lysate (denatured)
Preparation Method: Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 3.75 mg/ml, and then mixed with 5X Sample Buffer to become final 3 mg/ml in 1X Sample Buffer. The lysate was heated at 95°C for 5 min, and cooled rapidly.
Quantity: 200 μg
Host Species: Rat
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Spleen
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In ready-to-use 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue).
Concentration: 3 mg/mL
For Use With (Application): Immunoprecipitation, Western Blot
Description: Whole tissue lysate (non-denatured)
Preparation Method: Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml.
Quantity: 200 μg
Host Species: Rat
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: Spleen
Lysis Buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In modified RIPA Lysis Buffer.
Concentration: 5 mg/mL
For Use With (Application): __
Description: Mouse polyclonal antibody raised against a full-length human GALNT3 protein.
Preparation Method: __
Quantity: 50 μg
Host Species: Mouse
Content And Storage: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Tissue: __
Lysis Buffer: __
Quality Control Testing: __
Storage Buffer: __
Concentration: __
