Abnova™ 293T Whole Cell Lysate (Denatured)
Manufacturer: Abnova™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 89-932-019-Each-of-1 | In Stock | ₹ 20,791.08 |
89-932-019 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 20,791.08
GST (18%): ₹ 3,742.394
Total Price: ₹ 24,533.474
For Use With (Application)
Western Blot
Cell Line
293T
Lysis Buffer
1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Content And Storage
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Tissue
Kidney
Description
Whole cell lysate (denatured)
Preparation Method
Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.
Quantity
100 μL
Host Species
Human
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For Use With (Application): Western Blot
Cell Line: 293T
Lysis Buffer: 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Quality Control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer: In ready-to-use 1X Sample Buffer (0.2 M Tris-HCl, 2% SDS, 10% glycerol, 0.2% 2-mercaptoethanol, 0.2% Bromophenol blue).
Content And Storage: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Tissue: Kidney
Description: Whole cell lysate (denatured)
Preparation Method: Culture cells in 6-well plate to 80% confluent, remove media and then rinse with cold 1x PBS. Scrape cells off the plate surface and pipette cells into microcentrifuge tube to spin down the cells. Remove PBS and then lysis and resuspend cells with 205 ul 1X sample buffer. Mix well and heat at 90°C for 10 minutes, and then cooled rapidly.
Quantity: 100 μL
Host Species: Human
For Use With (Application): Western Blot
Cell Line: 293T
Lysis Buffer: __
Quality Control Testing: Transient overexpression cell lysate was tested with anti-76P antibody (Mfr. No. H00027229-B01) by Western Blot.
Storage Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: __
Description: TUBGCP4 293T Cell Transient Overexpression Lysate(Denatured)
Preparation Method: __
Quantity: 100 μL
Host Species: Human
For Use With (Application): Western Blot
Cell Line: 293T
Lysis Buffer: __
Quality Control Testing: Transient overexpression cell lysate was tested with anti-A2ML1 antibody (Mfr. No. H00144568-B01) by Western Blot.
Storage Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Content And Storage: Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Tissue: __
Description: A2ML1 293T Cell Transient Overexpression Lysate(Denatured)
Preparation Method: __
Quantity: 100 μL
Host Species: Human
