Gibco™ Rat BSEP Vesicles

Description

• Rat BSEP Vesicles are insect-derived purified plasma membranes with inserted BSEP transport protein (bile salt export pump, ABCB11)
• Our Rat BSEP Vesicles can be used to evaluate test compounds and drug interactions with BSEP in in vitro assays
• BSEP is an ATP-binding cassette (ABC) transporter that plays a key role in bile acid excretion from the liver
• Use Rat BSEP Vesicles to: Investigate the transporter interactions of your drug candidates Assess potential for transporter-mediated drug-drug interactions Obtain high quality results with a large signal to noise ratios Concept Behind ABC Transporter Vesicles ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential
• While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles
• It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles
• Clear and Reliable Results Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter
• Transporter Vesicle Details Rat BSEP Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles
• They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing BSEP
• Evaluation of the inhibitory activity of newly developed compounds towards BSEP-mediated transport of bile acids (e.g
• taurocholate) may give an indication of potential hepatotoxicity of the compounds.