Gibco™ Human MRP8 Vesicles

Description

• Human MRP8 Vesicles are insect derived purified plasma membranes with inserted MRP8 transport protein (Multidrug Resistance-associated Protein 8, ABCC11)
• Our Human MRP8 Vesicles can be used to evaluate test compounds and drug interactions with MRP8 in in vitro assays
• MRP8 is an ATP-binding cassette (ABC) transporter that carries organic anions
• Use Human MRP8 Vesicles to: Investigate the transporter interactions of your drug candidates Assess potential for transporter-mediated drug-drug interactions Obtain high quality results with a large signal to noise ratios Concept Behind ABC Transporter Vesicles ABC transporter vesicles are easy-to-use, efficient reagents for early assessment of a drug candidate's substrate and drug interaction potential
• While ABC transporters typically mediate the export of substrates out of cells, transporters expressed on these inside-out vesicles import substrates into the vesicles
• It is therefore possible to quantitatively evaluate transport activity for your compound by determining the amount incorporated into the vesicles
• Clear and Reliable Results Prepared from Sf9 cells which have been engineered to over-express specific ABC transporters, these 'inside-out' vesicles provide high levels of transporter activity with low background, giving you a clear signal if your compound is a substrate or inhibitor of a specific efflux transporter
• Transporter Vesicle Details Human MRP8 Vesicles are part of a larger group of transporter vesicles known as ABC transporter vesicles
• They are prepared from purified plasma membranes isolated from an insect cell system (Sf9 cells transfected with baculovirus) expressing MRP8
• MRP8 is highly expressed in the liver, where it may be associated with drug excretion
• It has been reported that MRP8 expression is increased in lung cancer, and the MRP8 gene may be involved in multidrug resistance to anti-cancer drugs.