NB800PC10
Novus Biologicals™ MEF Whole Cell Lysate
Manufacturer: Novus Biologicals™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NB800PC10-Each-of-1 | In Stock | ₹ 11,396.59 |
NB800PC10 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 11,396.59
GST (18%): ₹ 2,051.386
Total Price: ₹ 13,447.976
For Use With (Application)
Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
Test Specificity
MEF (Mouse Embryonic Fibroblast) whole cell extract lysate
Content And Storage
Store at -70°C. Avoid freeze-thaw cycles.
Tissue
Bone/Cartilage
Purity
Multi-step
Cellular Fraction
Whole
Related Products
Description
- Description The MEF Whole Cell Lysate is derived from mouse
- It has been validated for the following applications: Western Blot, SDS-PAGE
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For Use With (Application): Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
Test Specificity: MEF (Mouse Embryonic Fibroblast) whole cell extract lysate
Content And Storage: Store at -70°C. Avoid freeze-thaw cycles.
Tissue: Bone/Cartilage
Purity: Multi-step
Cellular Fraction: Whole
For Use With (Application):
Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
Test Specificity:
MEF (Mouse Embryonic Fibroblast) whole cell extract lysate
Content And Storage:
Store at -70°C. Avoid freeze-thaw cycles.
Tissue:
Bone/Cartilage
Purity:
Multi-step
Cellular Fraction:
Whole
For Use With (Application): Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) 10% (v/v) Glycerol
Test Specificity: Cell Line: Chinese Hamster Ovary (CHO)
Content And Storage: Store at -70°C. Avoid freeze-thaw cycles.
Tissue: Reproductive
Purity: Multi-step
Cellular Fraction: Whole
For Use With (Application):
Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) 10% (v/v) Glycerol
Test Specificity:
Cell Line: Chinese Hamster Ovary (CHO)
Content And Storage:
Store at -70°C. Avoid freeze-thaw cycles.
Tissue:
Reproductive
Purity:
Multi-step
Cellular Fraction:
Whole
For Use With (Application): Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) 10% (v/v) Glycerol
Test Specificity: Normal Mouse Brain whole cell extract lysate
Content And Storage: Store at -70°C. Avoid freeze-thaw cycles.
Tissue: Nervous
Purity: Multi-step
Cellular Fraction: Whole
For Use With (Application):
Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) 10% (v/v) Glycerol
Test Specificity:
Normal Mouse Brain whole cell extract lysate
Content And Storage:
Store at -70°C. Avoid freeze-thaw cycles.
Tissue:
Nervous
Purity:
Multi-step
Cellular Fraction:
Whole
For Use With (Application): Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
Test Specificity: Normal Mouse Liver whole cell extract lysate
Content And Storage: Store at -70°C. Avoid freeze-thaw cycles.
Tissue: Digestive/Waste
Purity: Multi-step
Cellular Fraction: Whole
For Use With (Application):
Ready-to-Use Lysates Are Especially Prepared as Positive Controls for Separation by SDS-PAGE and Subsequent Western Blot Analysis. Lysates Are Prepared in Denaturing Buffer WITHOUT Dissociating Agents (i.e. no 2-Mercaptoethanol or Dithiothreitol Has Been Added). Heat Lysate to 95 C for 5 Minutes and Rapidly Cool. If Dissociating Conditions Are Desired, Add Reducing Agent Prior to Heating. The Recommended Loading Volume / Lane is 10-20 μL Depending on The Size Format of Your Gel.
Storage Buffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
Test Specificity:
Normal Mouse Liver whole cell extract lysate
Content And Storage:
Store at -70°C. Avoid freeze-thaw cycles.
Tissue:
Digestive/Waste
Purity:
Multi-step
Cellular Fraction:
Whole